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Orientador(es)
Resumo(s)
The purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the
quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material
was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age.
The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for
missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation
of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using
photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all
four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics
of the analyzed semen of Holstein-Friesian bulls.
Descrição
Palavras-chave
AQP7 gene Holstein-Friesian bulls AQP7 polymorphism Reproduction Sperm cells quality Aquaporins Aquaglycero-porins Single nucleotide polymorphisms
Contexto Educativo
Citação
Kowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Cantalapiedra, Jesús Juan; Cerqueira, J.O.L.;Araújo, José Pedro. (2023). Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls. Zoological Society of Pakistan. ISSN 00309923. 55:1, p. 1-6
Editora
Elsevier