Percorrer por autor "Inumaro, Rodrigo Sadao"
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- Antioxidant Activity, Antiproliferative Activity, Antiviral Activity, NO Production Inhibition, and Chemical Composition of Essential Oils and Crude Extracts of Leaves, Flower Buds, and Stems of Tetradenia ripariaPublication . Sena, Jéssica da Silva; Rodrigues, Selma Alves; Sakumoto, Karina; Inumaro, Rodrigo Sadao; González-Maldonado, Pamela; Mendez-Scolari, Emilio; Piau, Ranulfo; Gonçalves, Daniela Dib; Mandim, Filipa; Vaz, Josiana A.; Gonçalves, José Eduardo; Sotelo, Pablo Hernan; Valle, Juliana Silveira do; Gazim, Zilda CristianiThe chemical composition of extracts (CEs) and essential oils (EOs) from Tetradenia riparia leaves, flower buds, and stems was analyzed. Antiproliferative activity against tumor cell lines, NO production inhibition, and antioxidant and antiviral activities were assessed. The CEs contained flavonoids, phenolic acids, coumarins, and saturated fatty acids. The EOs included monoterpenes, oxygenated sesquiterpenes, and diterpenes. NO production inhibition ranged from 76 to 247 μg mL−1, and antiproliferative activity exhibited GI50 between 20 and >204 μg mL−1, with low cytotoxicity (SI: 1.08 to 4.75). Reactive oxygen species inhibition ranged from 45 to 82%. Antioxidant activity varied when determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (IC50: 0.51 to 8.47 mg mL−1) and ferric reducing antioxidant power (0.35 to 0.81 μM ferrous sulfate per mg). The reduction in β-carotene–linoleic acid co-oxidation varied between 76.13 and 102.25%. The total phenolic content of CEs and EOs was 10.70 to 111.68 μg gallic acid mg−1. Antiviral activity against herpes simplex virus type 1 (HSV-1) showed an EC50 between 9.64 and 24.55 μg mL−1 and an SI between 8.67 and 15.04. Leaf EOs exhibited an EC50 of 9.64 μg mL−1 and an SI of 15.04. Our study unveils the diverse chemical composition and multifaceted pharmacological properties of T. riparia, demonstrating its potential as a valuable source of bioactive compounds for therapeutic applications.
- Cellular antioxidant, anti-inflammatory, and antiproliferative activities from the flowers, leaves and fruits of Gallesia integrifolia spreng harmsPublication . Silva, Gabriela Catuzo Canonico; Machado, Mariane de Almeida; Sakumoto, Karina; Inumaro, Rodrigo Sadao; Gonçalves, José Eduardo; Mandim, Filipa; Vaz, Josiana A.; Valle, Juliana Silveira do; Faria, Maria Graciela Iecher; Ruiz, Suelen Pereira; Piau Junior, Ranulfo; Gonçalves, Daniela Dib; Gazim, Zilda CristianiGallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2 & PRIME;-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI(50) between 51 and 230 & mu;g/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 & mu;g/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.
- Supercritical fluid-assisted extraction of hop cone residue from craft breweriesPublication . Inumaro, Rodrigo Sadao; Casalvara, Rhaira Fernanda Ayoub; Corrêa, Rúbia C.G.; Barros, Lillian; Pereira, Carla; Calhelha, Ricardo C.; Heleno, Sandrina A.; Pimentel, Tatiana Colombo; Gonçalves, José Eduardo; Cardozo-Filho, LucioThis study aimed to use supercritical fluid-assisted extraction in hop cone residue from craft breweries and define the best extraction parameters. Yield, compounds extracted, in vitro chemical and cell-based antioxidant ac-tivities, and antimicrobial and cytotoxic potentials were evaluated. The variables studied were temperature (40-60 degrees C), pressure (10-20 MPa), and ethanol percentage as co-solvent (5-10%) using a Taguchi experimental design (11 runs). The extracts showed inhibitory action towards Gram-positive and Gram-negative bacteria, mainly Proteus mirabilis and Enterococcus faecalis (MICs = 5-10 mg/mL). Furthermore, up to the maximum tested concentration (400 & mu;g/mL), the extracts showed no hepatotoxicity on a healthy porcine liver cell line (PLP2), indicating safety for food and pharmacological applications. Lower temperatures (40 degrees C), pressures (10 MPa), and ethanol percentages (5%) resulted in higher concentrations of some compounds (alpha-copaene, & gamma;-elemene, & gamma;-muurolene, & alpha;-muurolene, caryophyllene oxide) and higher antioxidant activity in cell-based assays, but lower yields. At the same time, lower temperatures (40 degrees C) and pressures (10 MPa) but higher ethanol percentages (10%) resulted in higher concentrations of other compounds (nerolidyl acetate, linalyl isobutyrate, & alpha;-acorenol, and geranyl isovalerate) and increased antioxidant activity in chemical systems. Our results support that the hop cone residue can be used to obtain supercritical fluid-assisted extracts with biological potentialities that might virtually be upcycled into multifunctional ingredients for the food and pharmacological industries. Using 40 degrees C, 10 MPa, and 5 or 10% ethanol percentage as process parameters is advisable.