Browsing by Author "Haminiuk, Charles Windson Isidoro"
Now showing 1 - 9 of 9
Results Per Page
Sort Options
- Bioaccessibility Performance of Phenolic Compounds from Red Fruits During Simulated Gastrointestinal Digestion and Colonic FermentationPublication . Bortolini, Débora Gonçalves; Barros, Lillian; Maciel, Giselle Maria; Peralta, Rosane M.; Corrêa, Vanesa G.; Finimundy, Tiane Cristine; Haminiuk, Charles Windson IsidoroRed fruits are rich in phenolic compounds, particularly anthocyanins, which contribute to their vibrant colors and health benefits. However, anthocyanins are chemically sensitive during digestion, which affects their bioaccessibility. This study evaluated the impact of simulated gastrointestinal digestion on whole red fruits (oral, gastric, intestinal, and colonic phases). Total phenolic content (TPC) ranged from 8.60−50.41 mg GAE/g DW, total flavonoid content (TFC) from 1.66−10.67 mg CAT/g DW, and total monomeric anthocyanins (TMA) from 0.54−1.28 mg CYA/g DW. Antioxidant activity strongly correlated with TPC (r ≥ 0.91). High-phenolic fruits showed lower bioaccessibility compared with low-phenolic ones. HPLC-DAD-ESI/MSn identified glycosylated anthocyanins, which were highly sensitive; jaboticaba anthocyanins were nonbioaccessible, while 51.83% remained bioaccessible in raspberries. Colonic fermentation further reduced TPC and TFC, and anthocyanins were entirely degraded. These results highlight the need for innovative delivery systems to improve anthocyanin bioaccessibility and functional benefits.
- Bioactive profile of edible nasturtium and rose flowers during simulated gastrointestinal digestionPublication . Bortolini, Débora Gonçalves; Barros, Lillian; Maciel, Giselle Maria; Brugnari, Tatiane; Modkovski, Tatiani Andressa; Fachi, Mariana Millan; Pontarolo, Roberto; Pinela, José; Ferreira, Isabel C.F.R.; Haminiuk, Charles Windson Isidoronot widely explored. The gastrointestinal environment can modify these compounds, resulting in new combinations with different bioactivity. This study aimed to evaluate the effects of simulated gastrointestinal digestion (SGD) on rose and nasturtium flower extracts. Using UPLC-HRMS, 38 phenolic compounds were identified, and the SGD caused significant changes, mainly in the glycosylated phenolic. Furthermore, antioxidant activity was correlated with the increase in the concentrations of some polyphenols. Tested Gram-negative bacteria showed sensitivity to the flower extracts; their growth was inhibited by up to 82.7%. SGD interrupted the bacterial growth inhibition power of the rose extracts. On the other hand, an increase in inhibition ranging from 52.25 to 54.72% was found for nasturtium extracts, correlated to the behavior of some bioactive. Hence, SGD resulted in significant changes in phenolic profiles of the edible flowers, increasing antioxidant activity and changing antimicrobial effects.
- Biosorption of bioactive compounds in bacterial nanocellulose: mechanisms and physical-chemical propertiesPublication . Fernandes, Isabela de Andrade Arruda; Maciel, Giselle Maria; Ribeiro, Isabela Sampaio; Pedro, Alessandra Cristina; Bortolini, Débora Gonçalves; Ribeiro, Valeria Rampazzo; Barros, Lillian; Haminiuk, Charles Windson IsidoroBacterial cellulose (BC) is a biomaterial produced by Gluconacetobacter xylinus, with wide applicability in different areas, such as biomedical, pharmaceutical, and food. BC production is usually carried out in a medium containing phenolic compounds (PC), such as teas, however, the purification process leads to the loss of such bioactive. Thus, the innovation of this research consists of the reincorporation of PC after the purification of the BC matrices through the biosorption process. In this context, the effects of the biosorption process in BC were evaluated to maximize the incorporation of phenolic compounds from a ternary mixture of hibiscus (Hibiscus sabdariffa), white tea (Camellia sinensis), and grape pomace (Vitis labrusca). The biosorbed membrane (BC-Bio) showed a great concentration of total phenolic compounds (TPC = 64.89 mg L-1) and high antioxidant capacity through different assays (FRAP: 130.7 mg L-1, DPPH: 83.4 mg L-1, ABTS: 158.6 mg L-1, TBARS: 234.2 mg L-1). The physical tests also indicated that the biosorbed membrane presented high water absorption capacity, thermal stability, low permeability to water vapor and improved mechanical properties compared to BC-control. These results indicated that the biosorption of phenolic compounds in BC efficiently increases bioactive content and improves physical membrane characteristics. Also, PC release in a buffered solution suggests that BC-Bio can be used as a polyphenol delivery system. Therefore, BC-Bio is a polymer with wide application in different industrial segments.
- Estudo da estabilidade e atividade biológica de bagaço de uva (Merlot) após simulação da digestão gastrointestinal e fermentação colónicaPublication . Corrêa, Rúbia C.G.; Haminiuk, Charles Windson Isidoro; Barros, Lillian; Dias, Maria Inês; Calhelha, Ricardo C.; Kato-Schwartz, Camila Gabriel; Correa, Vanesa G.; Peralta, Rosane M.; Ferreira, Isabel C.F.R.A produção de vinho representa hoje em dia uma das maiores atividades agroindustriais a nível mundial. Como consequência gera uma enorme quantidade de subprodutos (bagaço e borras) que representam por vezes até 30% das uvas vinificadas, sendo a maioria completamente descartados sem tratamento adequado e/ou outras finalidades [1-4]. O bagaço de uva representa, por isso, um abundante e acessível subproduto industrial que contém uma grande variedade de compostos bioativos, nomeadamente fenólicos, que têm sido relacionados com benefícios para a saúde do consumidor [l]. O objetivo deste estudo foi mimetizar a digestão gastrointestinal e a fermentação colónica de bagaço de uva de Merlot, de forma a avaliar uma possível redução do conteúdo em fitoquímicos, correlacionando o perfil de compostos fenólicos com as suas atividades biológicas. Assim, foram caracterizados três extratos de bagaço (inicial, digerido e fermentado) relativamente ao seu conteúdo em compostos fenólicos antociânicos e não antociânicos por HPLC-DAD-ESI/MS. Além disso, foi também a valiado o seu potencial antioxidante, antibacteriano e citotóxico para células tumorais e não-tumorais. Os compostos fenólicos mais abundantes identificados nas três amostras estudadas foram um dímero de (epi)catequina tipo B, (+)-catequina e (-)-epicatequina. Foram identificados vinte compostos fenólicos não antociânicos na amostra inicial (66 mg/g de extrato), tendo sido significativamente reduzidos para 11 compostos após digestão invitro. Foram identificadas cinco antocianinas, no entanto, as quantidades diminuíram imenso após digestão e fermentação. Pelos resultados obtidos podemos concluir que o processo de digestão in vitro promoveu drásticas reduções qualitativas e quantitativas no perfil de compostos fenólicos no extrato micial de bagaço de uvas Merlot. Tais alterações podem estar relacionadas com a diminuição de algumas bioatividades do extrato, como é o caso das propriedades antioxidantes e antibacterianas, embora não de forma diretamente proporcional. No entanto, a fermentação colónica teve um efeito positivo sobre o potencial citoóxico do extrato em linhas celulares tumorais humanas. Há ainda um conhecimento muito escasso sobre a estabilidade dos compostos fenólicos de Bagaço de uva e propriedades bioativas durante a digestão gastrointestinal e fermentação colónica, no entanto, os resultados obtidos poderão ser de grande utilidade para o desenvolvimento de novos suplementos nutracêuticos e produtos alimentares funcionalizados.
- Merlot grape pomace hydroalcoholic extract improves the oxidative and inflammatory states of rats with adjuvant-induced arthritisPublication . Gonçalves, Geferson A.; Soares, Andréia A.; Corrêa, Rúbia C.G.; Barros, Lillian; Haminiuk, Charles Windson Isidoro; Peralta, Rosane M.; Ferreira, Isabel C.F.R.; Bracht, AdelarGrape pomace is an agro-industrial residue produced worldwide. The purpose of this study was to identify and quantify the main phenolics present in an hydroalcohlic extract of a Merlot grape pomace and to investigate its effect on the oxidative and inflammatory states of adjuvant-induced arthritic rats. Daily doses of 250 mg of the extract per kg body weight were administered during 23 days. Several oxidative stress indicators in arthritic rats were maintained at their normal or closely normal levels in the plasma, liver and brain by the treatment. Additionally, the grape pomace also showed significant anti-inflammatory effects. From the 25 phenolics identified in the grape pomace extract the most abundant ones were catechin and catechin derivatives, which are possibly the most important antioxidant agents. The results suggest a potential applicability of the Merlot grape pomace hydroalcoholic extract in the improvement of the oxidative and inflammatory states in arthritic patients.
- New phytochemicals as potential human anti-aging compounds: reality, promise, and challengesPublication . Corrêa, Rúbia C.G.; Peralta, Rosane M.; Haminiuk, Charles Windson Isidoro; Maciel, Giselle Maria; Bracht, Adelar; Ferreira, Isabel C.F.R.Aging is an inevitable process influenced by genetic, lifestyle, and environmental factors. Indirect evidence shows that several phytochemicals can have anti-aging capabilities, although direct evidence in this field is still limited. This report aims to provide a critical review on aspects related to the use of novel phytochemicals as anti-aging agents, to discuss the obstacles found when performing most anti-aging study protocols in humans, and to analyze future perspectives. In addition to the extensively studied resveratrol, epicatechin, quercetin, and curcumin, new phytochemicals have been reported to act as anti-aging agents, such as the amino acid L-theanine isolated from green tea, and the lignans arctigenin and matairesinol isolated from Arctium lappa seeds. Furthermore, this review discusses the application of several new extracts rich in phytochemicals with potential use in anti-aging therapies. Finally, this review also discusses the most important biomarkers to test anti-aging interventions, the necessity of conducting epidemiological studies and the need of clinical trials with adequate study protocols for humans.
- Potential anti-diabetic properties of Merlot grape pomace extract: an in vitro, in silico and in vivo study of α-amylase and α-glucosidase inhibitionPublication . Kato-Schwartz, Camila Gabriel; Corrêa, Rúbia C.G.; Lima, Diego de Souza; Sá-Nakanishi, Anacharis B. de; Gonçalves, Geferson A.; Seixas, Flávio Augusto Vicente; Haminiuk, Charles Windson Isidoro; Barros, Lillian; Ferreira, Isabel C.F.R.; Bracht, Adelar; Peralta, Rosane M.A practical approach to control glycemia in diabetes is to use plant natural products that delay hydrolysis of complex sugars and promote the diminution of the release of glucosyl units into the blood plasma. Polyphenolics have been described as being effective in inhibiting amylases and α-glucosidases. Grape pomace is an important sub product of the wine industry, still rich in many compounds such as polyphenolics. In this context, the purpose of this study was to search for possible effects of a grape pomace extract on salivary and pancreatic α-amylases and α-glucosidase, as well as on intestinal glucose absorption. The Merlot grape pomace extract (MGPE) was prepared using a hydroalcoholic mixture (40% ethanol + 60% water). In vitro inhibition was quantified using potato starch (for amylases) and maltose (for α-glucosidase) as substrates. In vivo inhibition was evaluated by running starch and maltose tolerance tests in rats with or without administration of MGPE. Ranking of the extract compounds for its affinity to the α-amylases was accomplished by computer simulations using three different programs. Both α-amylases, pancreatic and salivary, were inhibited by the MGPE. No inhibition on α-glucosidase, however, was detected. The IC50 values were 90 ± 10 μg/mL and 143 ± 15 μg/mL for salivary and pancreatic amylases, respectively. Kinetically this inhibition showed a complex pattern, with multiple binding of the extract constituents to the enzymes. Furthermore, the in silico docking simulations indicated that several phenolic substances, e.g., peonidin-3-O-acetylglucoside, quercetin-3-O-glucuronide and isorhamnetin-3-O-glucoside, besides catechin, were the most likely polyphenols responsible for the α-amylase inhibition caused by MGPE. The hyperglycemic burst, an usual phenomenon that follows starch administration, was substantially inhibited by the MGPE. Our results suggest that the MGPE can be adequate for maintaining normal blood levels after food ingestion.
- Stability and biological activity of Merlot (Vitis vinifera) grape pomace phytochemicals after simulated in vitro gastrointestinal digestion and colonic fermentationPublication . Corrêa, Rúbia C.G.; Haminiuk, Charles Windson Isidoro; Barros, Lillian; Dias, Maria Inês; Calhelha, Ricardo C.; Kato-Schwartz, Camila Gabriel; Correa, Vanesa G.; Peralta, Rosane M.; Ferreira, Isabel C.F.R.Grape pomace is an abundant/accessible food industry by-product that contains a wide range of phenolic compounds, which have been related to several health benefits and bioactivities. The aim of this study was to mimic the gastrointestinal digestion and the colonic fermentation of Merlot grape pomace, in order to unravel possible phytochemical contents reductions and the processes associated with them, as a tentative to relate the phenolic compound profiles of the extracts with their biological properties. LC-DAD-ESI/MS suggested that the in vitro digestion process promoted drastic qualitative and quantitative reductions in the phenolic compounds profile of the Merlot grape pomace crude extract. Such alterations could be related to the decreases of some bioactivities of the extract, which seems to be the case of antioxidant and antibacterial properties, although not in a directly proportional manner. However, the simulated colonic fermentation seems to have a positive effect over the extract's antiproliferative potential.
- The past decade findings related with nutritional composition, bioactive molecules and biotechnological applications of Passiflora spp. (passion fruit)Publication . Corrêa, Rúbia C.G.; Peralta, Rosane M.; Haminiuk, Charles Windson Isidoro; Maciel, Giselle Maria; Bracht, Adelar; Ferreira, Isabel C.F.R.Background: For centuries, Passiflora species have been used in folk medicine, especially as sedatives and anxiolytics, and in recent decades have been exploited by the food, pharmaceutical and cosmetics industries. Currently, the concentrated juice manufacture is the most impacting activity involving Passiflora spp., since its market is expanding worldwide. Such a wide-scale passion fruit processing inevitably ends up generating huge amounts of wastes (i.e., seeds, rinds and bagasse), as it corresponds to more than a half of the fruit mass. Scope and Approach: The present review aims to summarize and evaluate the past decade findings related with nutritional composition, bioactive molecules and biotechnological applications of Passiflora spp., with special attention to the potentialities of its co-products. Key Findings and Conclusions: A series of emerging technologies firmed on non-thermal principals, along with encapsulation by nanoparticles methods, have been studied for the sustainable recovery of some target high added-value compounds from Passiflora spp. and their co-products, which include biologically active components such as polyphenols, terpenes, peptides, polysaccharides and dietary fibers. In addition, the passion fruit co-products are promising (and still underutilized) agents of bioconversion and bioremediation, besides low-cost functional ingredients for both cosmetic and food industry.