Percorrer por autor "Duarte, S.C."
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- Influencing factors on bread-derived exposure to ochratoxin A: type, origin and compositionPublication . Duarte, S.C.; Bento, J.M.V.; Pena, A.; Lino, C.M.; Delerue-Matos, Cristina; Oliveira, Beatriz; Alves, M. Rui; Pereira, J.A.The nearly ubiquitous consumption of cereals all over the world renders them an important position in international nutrition, but concurrently allocates exposure to possible contained contaminants. Mycotoxins are natural food contaminants, difficult to predict, evade, and reduce, so it is important to establish the real contribution of each contaminated food product, with the aim to evaluate mycotoxin exposure. This was the key objective of this survey and analysis for ochratoxin A content on 274 samples of commercialized bread in the Portuguese market, during the winter 2007. Different bread products were analyzed through an HPLC-FD method, including traditional types, novel segments, and different grain based bread products. A wide-ranging low level contamination was observed in all regions and types of bread products analyzed, especially in the Porto and Coimbra regions, and in the maize and whole-grain or fiber-enriched bread. Nevertheless, the exposure through contaminated wheat bread continues to be the most significant, given its high consumption and dominance in relation to the other types of bread.
- Monitoring of ochratoxin a exposure of the Portuguese population through a nationwide urine survey – Winter 2007Publication . Duarte, S.C.; Bento, J.M.V.; Pena, A.; Lino, C.M.; Delerue-Matos, Cristina; Oliva-Teles, T.; Morais, S.; Correia, Manuela; Oliveira, Beatriz; Alves, M. Rui; Pereira, J.A.Ochratoxin A (OTA) is a mycotoxin produced by a variety of fungi, such as Penicillium verrucosum and Aspergillium spp., which has been found to have a wide number of potentially deadly toxic effects, and can enter the human organism through a variety of means. It then finds its way into the bloodstream and, after a lengthy process, is eventually excreted through the urine. It can thus be detected in its original form not only in blood samples but also in this biological medium. As such, and in an attempt to evaluate the exposure of the Portuguese population to this mycotoxin, morning urine samples were collected during the Winter of 2007, from each of five geographically distinct Portuguese locations — Bragança, Porto, Coimbra, Alentejo, and Algarve — and subjected to extraction by immunoaffinity columns and to OTA quantification through liquid chromatography coupled with fluorescence detection. Prevalent incidence was higher than 95% with Coimbra being the exception (incidence of 73.3%). In nearly all locations, the OTA content of most samples was found to be above the limit of quantification (LOQ) of 0.008 ng/ml. Indeed, excluding Coimbra, with an OTA content level of 0.014 ng/ml, all regions featured content values over 0.021 ng/ml.
- Ochratoxin A contamination of bread – Portugal nationwide survey during winter 2007/2008.Publication . Duarte, S.C.; Bento, J.M.V.; Pena, A.; Lino, C.M.; Pereira, J.A.; Delerue-Matos, Cristina; Oliva-Teles, T.; Morais, S.; Correia, Manuela; Oliveira, BeatrizOchratoxin A (OTA) remains one of the most important mycotoxins known, due to its ubiquitous occurrence, wide range of susceptible food commodities and observed toxic effects, in both animals and humans. The reported toxic effects include carcinogenic, nephrotoxic, teratogenic, neurotoxic and immunotoxic.
- Ochratoxin A content in urine from Bragança and Alentejo: a compartive analysis (Winter 2007).Publication . Duarte, S.C.; Bento, J.M.V.; Pena, A.; Lino, C.M.; Pereira, J.A.; Delerue-Matos, Cristina; Oliva-Teles, T.; Morais, S.; Correia, Manuela; Oliveira, BeatrizOchratoxin A (OTA) is a mycotoxin which possesses a variety of toxic effects, including enzyme inhibition, immunosuppression, teratogenicity, nephrotoxicity, and carcinogenicity. It is produced by fungi for which foodstuffs such as beans, cereals, fruits, and seeds constitute an ideal growing media. It has proven itself at least partly resistant to food processing methods, meaning it is also present in derived products and thus finds its way into the human organism. Recent studies have suggested that, though OTA can be found in both plasma and urine – through which it is eliminated, though with great difficulty – the latter provides a better indication of OTA ingestion. Its collection procedure is also less invasive, and developments in analytical methodology allow an equally precise analysis.