Percorrer por autor "Cerqueira, J.O.L."
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- Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bullsPublication . Kowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Cantalapiedra, Jesús J.; Cerqueira, J.O.L.; Araújo, José PedroThe purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.