Browsing by Author "Campos, Jaqueline Ferreira"
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- Antimicrobial activity of propolis from the Brazilian stingless bees Melipona quadrifasciata anthidioides and Scaptotrigona depilis (Hymenoptera, Apidae, Meliponini)Publication . Campos, Jaqueline Ferreira; Bonamigo, Thaliny; Rocha, Paola dos Santos; Paula, Vanessa B.; Santos, Uilson Pereira dos; Balestieri, José Benedito Perrella; Silva, Denise Brentan; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Souza, Kely de Picoli; Santos, Edson Lucas dosMelipona quadrifasciata anthidioides and Scaptotrigona depilis are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions and evaluate the antimicrobial activity of propolis produced by M. q. anthidioides and S. depilis. The ethanolic extracts of propolis of M. q. anthidioides (EEP-M) and S. depilis (EEP-S) were prepared, and their chemical constituents were characterized by HPLC-ESI-MS. The antimicrobial activity was evaluated against bacteria and fungi, isolated from reference strains and hospital origin resistant to the action of antibiotics. From EEP-M, phenolic compounds were annotated, including gallic acid, ellagic acid, and flavonoids, as well as diterpenes and triterpenes. EEP-S showed mainly triterpene in its chemical composition. Both extracts inhibited the growth of medically relevant bacteria and fungi, including hospital-acquired and antimicrobial-resistant. In general, EEP-S showed better antimicrobial activity compared to EEP-M. The MIC of EEP-S against vancomycin-resistant Enterococcus faecalis was 3.50 mg/mL, while the MIC of EEP-M was 5.33 +/- 0.16 mg/mL. In conclusion, this study shows that propolis produced by M. q. anthidioides and S. depilis has the potential to be used for the prevention or treatment of microbial infections.
- Antimicrobial, Antioxidant, Anti-Inflammatory, and Cytotoxic Activities of Propolis from the Stingless Bee Tetragonisca fiebrigi (Jataí)Publication . Campos, Jaqueline Ferreira; Santos, Uilson Pereira dos; Rocha, Paola dos Santos; Damião, Marcio José; Balestieri, José Benedito Perrella; Cardoso, Claudia Andréa Lima; Paredes-Gamero, Edgar Julian; Estevinho, Leticia M.; Souza, Kely de Picoli; Santos, Edson Lucas dosPropolis from stingless bees Tetragonisca fiebrigi found in Brazil is used in folk medicine by their nutritional and therapeutic properties. However, there are no scientific records evidencing such properties. The present study was designed to investigate the chemical composition and the biological properties of propolis from T. fiebrigi. For this, the chemical composition of the ethanol extract of propolis (EEP) was determined by GC-MS and presented phenolic compounds, alcohol, and terpenes as its major class compounds. The antimicrobial activity was accessed in gram-positive and gram-negative bacteria and in fungi, isolated from different biological fluids and reference strains. The EEP was active against all microorganisms and showed antioxidant activity by scavenging free radicals, inhibiting hemolysis and lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The anti-inflammatory potential of the EEP was confirmed by inhibition of the hyaluronidase enzyme. The cytotoxic activity was concentration-dependent against K562 cells, with a predominance of death by necrosis. Taken together, these results show that propolis from T. fiebrigi has important therapeutic activities, which suggest its potential application in the pharmaceutical industry, as well as in health foods, beverages, and nutritional supplements.
- Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa gomesPublication . Santos, Uilson Pereira dos; Campos, Jaqueline Ferreira; Torquato, Heron F. Vieira; Paredes-Gamero, Edgar Julian; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Souza, Kely de Picoli; Santos, Edson Lucas dosThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-diphenyl-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2'-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis.
- Atividade antimutagênica de geoprópolis de melípona quadrifasciata anthidioides (HYMENOPTERA, APIDAE)Publication . Campos, Jaqueline Ferreira; Santos, Cintia Miranda; Santos, H.S.; Balestieri, José Benedito Perrella; Estevinho, Leticia M.; Sousa, Kelly de Picoly; Santos, Edson Lucas dosO câncer é caracterizado por distúrbios na proliferação e diferenciação celular, apresentando causas diversas e complexas. A patogenia molecular da maioria dos cânceres é atribuída a lesões ou mutações genéticas, resultando em graves alterações celulares. Assim, a busca por compostos com potencial antimutagênico é crescente, os quais podem reduzir ou reverter os danos no DNA. Neste contexto, o objetivo deste estudo foi investigar a atividade antimutagênica do extrato hidroetanólico de geoprópolis da abelha sem ferrão Melipona quadrifasciata anthidioides. Metodologia: O extrato hidroetanólico de geoprópolis (EHGP) foi preparado a partir da mistura de 80 g de geoprópolis em 240 mL de etanol 70%, sob agitação por 24 horas a temperatura ambiente. Posteriormente, a solução foi filtrada, rotaevaporada e liofilizada. Para determinar a atividade antimutagênica do EHGP, foram utilizadas células de Saccharomyces cerevisiae ATCC 201137 (linhagem D7 diploide) as quais foram usadas para determinar a frequência de convertentes espontâneas no locus de triptofano (trp) e revertentes no locus de isoleucina (ilv). As células foram plaqueadas a 2 x 108 células/mL e incubadas com o composto mutagênico (ethyl methanesulfonate) e o EHGP (0,15 e 0,30 %) por 2 h a 37 °C. Em seguida, as células foram transferidas para meio seletivo para determinar a sobrevivência, trp convertentes e ilv revertentes. Resultados: O EHGP reduziu a sobrevivência da S. cerevisiae D7 em torno de 50%, por sua ação fungicida, e apresentou efeito antimutagênico inibindo os danos promovidos pelo composto mutagênico. O extrato apresentou redução das frequências de conversão de gene em 30,7 ± 4,8 e 41,5 ± 1,7 %, nas concentrações de 0,15 e 0,30 %, respectivamente. Adicionalmente, reduziu significativamente o número de colônias mutantes em 79,4 ± 0,8 (concentração de 0,15 %) e 89,3 ± 0,5 % (concentração de 0,30 %).Conclusão: Em suma, os resultados obtidos mostram que o extrato de geoprópolis de Melipona quadrifasciata anthidioides apresenta potencial em inibir lesões ou mutações genéticas induzidas por compostos mutagênicos em leveduras.
- Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 micePublication . Rocha, Paola dos Santos; Orué, Sarah Lam; Leite, Daniel Ferreira; Espindola, Priscilla Pereira de Toledo; Cassemiro, Nadla Soares; Silva, Denise Brentan; Carollo, Carlos Alexandre; Nunes-Souza, Valéria; Rabelo, Luiza Antas; Campos, Jaqueline Ferreira; Estevinho, Leticia M.; Santos, Edson Lucas dosObesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life.
- Chemical composition and pharmacological effects of geopropolis produced by Melipona quadrifasciata anthidioides.Publication . Santos, Cintia Miranda; Campos, Jaqueline Ferreira; Santos, Jaqueline Freitas dos; Balestieri, José Benedito Perrella; Silva, Denise Brentan; Souza, Kely de Picoli; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Santos, Edson Lucas dosStingless bees produce geopropolis, which is popularly described for its medicinal properties, but for which few scientific studies have demonstrated pharmacological effects. The objective of this study was to investigate the chemical composition of the geopropolis of Melipona quadrifasciata anthidioides and to evaluate its antioxidant, antimutagenic, anti-inflammatory, and antimicrobial activities. The composition of the hydroethanolic extract of geopropolis (HEG) included di- and trigalloyl and phenylpropanyl heteroside derivatives, flavanones, diterpenes, and triterpenes. HEG showed antioxidant action via the direct capture of free radicals and by inhibiting the levels of oxidative hemolysis and malondialdehyde in human erythrocytes under oxidative stress. HEG also reduced the frequency of gene conversion and the number of mutant colonies of S. cerevisiae. The anti-inflammatory action of HEG was demonstrated by the inhibition of hyaluronidase enzyme activity. In addition, HEG induced cell death in all evaluated gram-positive bacteria, gram-negative bacteria, and yeasts, including clinical isolates with antimicrobial drug resistance. Collectively, these results demonstrate the potential of M. q. anthidioides geopropolis for the prevention and treatment of various diseases related to oxidative stress, mutagenesis, inflammatory processes, and microbial infections.
- Chemical profile and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of geopropolis from the stingless bee Melipona orbignyiPublication . Santos, H.S.; Campos, Jaqueline Ferreira; Santos, Cintia Miranda; Balestieri, José Benedito Perrella; Silva, Denise Brentan; Carollo, Carlos Alexandre; Souza, Kely de Picoli; Estevinho, Leticia M.; Santos, Edson Lucas dosLicensee MDPI, Basel, Switzerland. Geopropolis is a resin mixed with mud, produced only by stingless bees. Despite being popularly known for its medicinal properties, few scientific studies have proven its biological activities. In this context, the objective of this study was to determine the chemical composition and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of the Melipona orbignyi geopropolis. The hydroalcoholic extract of geopropolis (HEGP) was prepared and its chemical composition determined by high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). The antioxidant activity was determined by the capture of free radicals and inhibition of lipid peroxidation in human erythrocytes. The anti-inflammatory activity was evaluated by the inhibition of the hyaluronidase enzyme and the antimutagenic action was investigated in Saccharomyces cerevisiae colonies. The antimicrobial activities were determined against bacteria and yeasts, isolated from reference strains and hospital origin. The chemical composition of HEGP included flavonoids, derivatives of glycosylated phenolic acids and terpenoids. HEGP showed high antioxidant activity, it inhibited the activity of the inflammatory enzyme hyaluronidase and reduced the mutagenic effects in S. cerevisiae. In relation to the antimicrobial activity, it promoted the death of all microorganisms evaluated. In conclusion, this study reveals for the first time the chemical composition of the HEGP of M. orbignyi and demonstrates its pharmacological properties.
- The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic EffectsPublication . Campos, Jaqueline Ferreira; Castro, David Tsuyoshi Hiramatsu; Damião, Marcio José; Torquato, Heron F. Vieira; Paredes-Gamero, Edgar Julian; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Souza, Kely de Picoli; Santos, Edson Lucas dosNatural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation.