Percorrer por autor "Boughanmi, Mohamed Belhassan"
A mostrar 1 - 2 de 2
Resultados por página
Opções de ordenação
- In silico characterization of molecular factors involved in metabolism and pathogenicity of Phytophthora cinnamomiPublication . Boughanmi, Mohamed Belhassan; Branco, Iuliia; Choupina, AltinoPhytophthora cinnamomi is classified as one of the most devastating plant pathogens in the world. It has a destructive effect on more than 5000 horticultural and forestry species in the world, and especially on Castanea sativa. The genus Phytophthora belongs to the Class Oomycetes, a group of fungus like organisms which provoke plant diseases via motile zoospores. Control of this organism is considered very challenging because of the limited range of effective chemical inhibitors. The development of sustainable control measures for the future management of P. cinnamomi requires in-depth knowledge of the cellular and molecular bases of development and metabolism. The aim of this review was to identify molecular factors associated with the metabolism of P. cinnamomi by studying the genes implicated in fundamental metabolism using tools of bioinformatics. Also, some genes involved in pathogenicity will be cited and characterized, such as genes coding for transglycosylases. Genomic sequences of P. cinnamomi were analyzed using an open reading frame (ORF) finder. The identified ORFs products (proteins) were compared to sequences already described and with known functions present in databases such as NCBI and fungi database. In this way, homologous proteins were found, with the respective specific domains, to proteins involved in the metabolism and pathogenicity of Phytophthora ssp.
- Molecular factors in the metabolism and pathogenicity of Phytophthora cinnamomi: characterization of RXLR family genes, Avr3a, and Avr1bPublication . Boughanmi, Mohamed Belhassan; Choupina, Altino; Rejeb, Ines BenPhytophthora cinnamomi, soil-borne pseudo fungi from the class of oomycete, not only representing a real threat to strategic cultures across the world but also in forestry. This pathogen is able to colonize roots of Castanea sativa causing ink disease. For that many studies are established to well understand the mechanism of infection by exogenous molecules secreted during contact with host cells. Those molecules have been identified thanks to the progress in molecular biology using technics like RNA Seq. The Avr like genes expresses an RXLR protein family (Arginine-X-leucine-Arginine; X could be any amino acid), implicated in the pathogenicity of several pseudo-fungi of the genus Phytophthora, especially in Phytophthora infestans, Phytophthora sojae and Phytophthora palmivora. In this work, we isolate sequence, and characterize the Avr3a and Avr1b homologs in P. cinnamomi using molecular biology techniques such as PCR. In addition, we identified, in genomic sequences deposited in the databases, molecular factors involved in the metabolism and pathogenicity of P. cinnamomi using bioinformatics tools. In order to better understand its role in the pathogenicity mechanism, interference RNA silencing cassettes were designed for the genes encoding AVR proteins.