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Browsing by Author "Bortolucci, Wanessa C."

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  • Cytotoxicity and anti-inflammatory activities of Gallesia integrifolia (Phytolaccaceae) fruit essential oil
    Publication . Bortolucci, Wanessa C.; Raimundo, Keila Fernanda; Fernandez, Carla Maria Mariano; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Barros, Lillian; Gonçalves, José Eduardo; Linde, Giani Andrea; Colauto, Nelson Barros; Gazim, Zilda Cristiani
    The chemical composition of Gallesia integrifolia fruits essential oil was obtained by hydrodistillation using a modified Clevenger apparatus was investigated by gas chromatography coupled to mass spectrometry (GC/MS). In addition, the cytotoxicity activity against human tumor cell lines MCF-7 (breast adenocarcinoma), NCI-H460 (large cell lung carcinoma), HeLa (cervical carcinoma), and HepG2 (hepatocellular carcinoma), and non-tumor PLP2 (porcine liver primary cells) as well as the anti-inflammatory action was proposal. The compounds were predominantly organosulfates (2,8-dithianonane, dimethyl trisulfide, and lenthionine). Anti-inflammatory activity that provides 50% inhibition of nitric oxide production (55 µg/mL) of essential oil of the fruits. It also presents cytotoxic activity against MCF-7 (GI50 = 66 µg/mL), NCI-H-460 (GI50 = 147 µg/mL), HeLa (GI50 = 182 µg/mL) and HepG2 (GI50 = 240 µg/mL). The essential oil is more active in tumor cells than in non-tumor cells and the GI50 values for essential oil reported in our work support future studies.
    2021Journal article Open access Show more
  • Lentinus crinitus basidiocarp stipe and pileus: chemical composition, cytotoxicity and antioxidant activity
    Publication . Bertéli, Míria B.D.; Oliveira Filho, Olavo B.Q.; Freitas, Janyeli D.S.; Bortolucci, Wanessa C.; Silva, Gustavo R.; Gazim, Zilda Cristiani; Lívero, Francislaine A.R.; Lovato, Evellyn C.W.; Valle, Juliana S.; Linde, Giani Andrea; Barros, Lillian; Reis, Filipa S.; Ferreira, Isabel C.F.R.; Paccola-Meirelles, Luzia D.; Colauto, Nelson Barros
    Lentinus crinitus is a wild fungus, which produces mushrooms consumed by some Amazonian Indians. Besides, it is recognized for its diverse biological activities and biotechnological applications. However, there are few reports with limited information on basidiocarp chemical composition and cytotoxicity. Our study determined and evaluated the chemical composition, cytotoxicity, and antioxidant activity of L. crinitus pileus and stipe separately. Chromatographic methods were used to evaluate basidiocarp chemical composition. Cytotoxicity was verified using a cell culture from porcine liver and against a panel of human tumor cells from different models. Antioxidant activity was assessed by different in vitro methods. The pileus had higher levels of protein, ash, tocopherols, and organic acids, mainly malic acid, than the stipe. The stipe revealed higher contents of carbohydrates, energy, soluble sugars, and phenolic acids, mostly p-hydroxybenzoic acid. L. crinitus basidiocarp has mainly trehalose as soluble sugar, and less than 1% fat being ~60% polyunsaturated fatty acids (mostly linoleic and oleic acids), and ~13% saturated fatty acids (mostly palmitic acid). L. crinitus revealed high antioxidant activity for most methods and no cytotoxic activity against tumor and non-tumor cells. L. crinitus basidiocarp can be considered a functional food with applicability in food, cosmetic, and pharmaceutical industries. Graphic abstract: [Figure not available: see fulltext.]
    2021Journal article Open access Show more