Percorrer por autor "Barros, M. Teresa"
A mostrar 1 - 4 de 4
Resultados por página
Opções de ordenação
- Antimicrobial and cytotoxic activities of 1,2,3-triazole-sucrose derivativesPublication . Petrova, Krasimira T.; Potewar, Taterao M.; Correia-da-Silva, Paula; Barros, M. Teresa; Calhelha, Ricardo C.; Ćirić, Ana; Soković, Marina; Ferreira, Isabel C.F.R.A library of 1-(1′,2,3,3′,4,4′,6-hepta-O-acetyl-6′-deoxy-sucros-6′-yl)-1,2,3-triazoles have been investigated for their antibacterial, antifungal and cytotoxic activities. Most of the target compounds showed good inhibitory activity against a variety of clinically and food contaminant important microbial pathogens. In particular, 1-(1′,2,3,3′,4,4′,6-hepta-O-acetyl-6′-deoxy-sucros-6′-yl)-4-(4-pentylphenyl)-1,2,3- triazole (5) was highly active against all the tested bacteria with minimal inhibitory concentrations (MICs) ranging between 1.1 and 4.4 μM and bactericidal concentrations (MBCs) from 2.2 and 8.4 μM. The compound 1-(1′,2,3,3′,4,4′,6-hepta-O-acetyl-6′-deoxy-sucros-6′-yl)-4-(4-bromophenyl)-1,2,3-triazole (3) showed antifungal activity with MICs from 0.6 to 4.8 μM and minimal fungicidal concentrations (MFCs) ranging between 1.2 and 8.9 μM. Furthermore, some of the compounds possessed moderate cytotoxicity against human breast, lung, cervical and hepatocellular carcinoma cell lines, without showing toxicity for nontumor liver cells. The above mentioned derivatives represent promising leads for the development of new generation of sugar-triazole anti fungal agents.
- Antimicrobial and cytotoxic activities of short carbon chain unsaturated sucrose estersPublication . Petrova, Krasimira T.; Barros, M. Teresa; Calhelha, Ricardo C.; Soković, Marina; Ferreira, Isabel C.F.R.A library of C3–C5 unsaturated 6-O-sucrose esters have been investigated for their antibacterial, antifungal, and cytotoxic activities. Most of the target compounds showed good inhibitory activity against a variety of clinically and food contaminant important microbial pathogens. In particular, 6-O-methacryloyl sucrose 2 and 1′,2,3,3′,4,4′,6′-hepta-O-acetyl-6-O-methacryloyl sucrose 9 were the most active bactericides against all the tested bacteria with minimal inhibitory concentrations (MICs) ranging between 0.24 and 1.40 μM. The compound 9 showed also the highest antifungal activity with MICs from 0.28 to 1.10 μM. The synthesized compounds possessed low cytotoxicity against human breast, lung, cervical, and hepatocellular carcinoma cell lines without showing toxicity for non-tumor liver cells. Thus, this library of short carbon chain unsaturated sucrose esters represent promising leads for the development of new generation of sucrose-based antimicrobial agents.
- Composición de los aditivos en embutido fermentado portugués (chouriço) después del secadoPublication . Pires, Preciosa; Araújo, José Pedro; Barros, M. Teresa; Fernandes, E.; Cerqueira, Joaquim; Cadavez, Vasco; Gonzales-Barron, Ursula; Dias, TeresaChouriço is a Portuguese dry-fermented sausage, which is much appreciated among consumers and highly consumed due to its organoleptic characteristics. Hence, the objective of this study was to investigate the food additive composition in dry products and comparison with the values in UE Regulation. The maximum limits allowed, according to Reg (EU) 1129/2011, are met for nitrite in both factories, with values well below the limit of 150 mg/kg (between 0,42-8,01 mg/kg). For nitrate salts the values are below the maximum limit in one production (between 10,1-22,6 mg/kg) but above the maximum level (> 150mg/kg) in another production (above 164,5 mg/kg). Regarding phosphates, both factories have their products not meeting the EU Reg (> 500 mg / kg). The chouriço is a meat product that can be consumed raw. To this product are added additives which act as antioxidants, in the case of nitrite, and as preventive or inhibitors of microbial growth. The concentrations of these salts have to be controlled, but its positive effects are superimposed on the possibility of problems originated with growth of microorganisms.
- Synthesis, characterization, antimicrobial and antitumor activities of sucrose Octa(N-ethyl)carbamatePublication . Raposo, Claudia; Petrova, Krasimira T.; Barros, M. Teresa; Calhelha, Ricardo C.; Soković, Marina; Ferreira, Isabel C.F.R.Sucrose octa(N-ethyl)carbamate was synthesized directly from sucrose and ethyl isocyanate, and its structure was confirmed by various analytical methods, such as 1H and 13C NMR, FTIR, m.p., MS, and optical rotation. Its antibacterial, antifungal and cytotoxic activities were investigated. It exhibited strong inhibition against all bacteria tested, namely S. aureus (MIC 0.18±0.006), B. cereus (MIC 0.094±0.000), M. flavus (MIC 0.28±0.01), L. monocytogenes (MIC 0.18±0.006), P. aeruginosa (MIC 0.094±0.002), S. typhimurium (MIC 0.094±0.002), E. coli (MIC 0.18±0.006) and E. cloacae (MIC 0.18±0.006) and strong antifungal activity towards T. viride (MIC 0.09 ± 0.006), A. versicolor (MIC 0.18 ± 0.01), A. ochraceus (MIC 0.375 ± 0.01) and P. ochrochloron (MIC 0.375 ± 0.04). Furthermore, it showed moderate antitumor potential against human breast (GI50357.20±14.12), colon (GI50 332.43±11.19) and cervical (GI50 282.67±3.97) cell lines and, more important, without hepatotoxicity.