Percorrer por autor "Babo, Pedro"
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- Effects of different treatments on the phenolic composition of acorn extracts from Quercus robur and Quercus rotundifoliaPublication . Alonso-Esteban, José Ignacio; Mateus, Cristiano; Finimundy, Tiane C.; Rodrigues, Daniele Bobrowski; Babo, Pedro; Canadas, Raphael; Barros, LillianThe European oak (Quercus robur L.) and the holm oak (Quercus rotundifolia Lam.) are two examples of endemic species that already contribute for the socio-economic development of the Iberian Peninsula. The first one stands out because of its wood, and the second one has been traditionally managed as savannah-like ecosystems for livestock breeding [1]. However, from a chemical perspective, the potential of these plants, especially their acorns, can be further expanded. For this work, acorn samples of the two mentioned species were submitted to four different extraction methods in order to characterize the phenolic compounds of each extract. The four extractions were the following: 1 – maceration with water at 40 ºC for one hour; 2 – infusion with boiling water for 5 minutes; 3 – decoction with boiling water for 5 minutes; 4 – maceration with ethanol 80% (v/v) at room temperature for 1 hour. The extractions were performed using a solid/liquid ratio of 25 g/L and the phenolic compounds were analyzed by HPLD-DAD-ESI/MS [2]. The average extraction yields ranged between 9.58 – 21.89 g of extract/100 g for Q. robur and from 2.88 to 6.82 g of extract/100 g for Q. rotundifolia. Regarding the composition of phenolic compounds, gallic and ellagic acids were the most abundant molecules found in the samples of these species [3]. The total content of phenolic compounds varied between 71 - 124 mg/g of extract and 70 – 90.5 mg/g of extract in Q. rotundifolia and Q. robur, respectively. Gallic acid was the compound which was found in the greatest concentration in all the extractions, reaching its maximum concentration when the aqueous maceration extraction was applied. Ellagic acid and its derivatives (ellagitannins) were also found in the extracts, but to a lesser extent than gallic acid. Considering the extraction yield and the content of phenolic compounds, the most promising extraction method was the aqueous maceration, which could be applied to obtain extracts rich in gallic acid and ellagitannins from acorns.
- Exploring acorn shells: Phenolic composition and bioactive potential for sustainable valorizationPublication . Mateus, Cristiano; Alonso-Esteban, José Ignacio; Finimundy, Tiane C.; Mandim, Filipa; Oliveira, Izamara; Babo, Pedro; Ferreira, Isabel C.F.R.; Barros, LillianPedunculate (Quercus robur L.), holm (Quercus rotundifolia Lam.), and cork (Quercus suber L.) oaks are abundant across the Portuguese landscape. This study aims to evaluate the phenolic composition and bioactivities of acorn shell samples and determine their potential as a functional compound source. In total, five acorn shell samples collected in different locations and from different species were analyzed: Q. rotundifolia (Q. rot-1 and Q. rot-2), Q. suber (Q. sub-1 and Q. sub-2) and Q. robur (Q. rob-1). A total of nine phenolic compounds were tentatively identified, namely gallic and ellagic acids and derivatives. Digalloyl hexoside was the compound detected in higher concentrations in all extracts (2.093 – 8.3 mg/g extract). Q. suber samples exhibited the lowest IC50 values for TBARS assay, lower than the positive control used (Trolox). Overall, the studied samples demonstrated the capacity to inhibit the proliferation of all tumor cell lines tested. Sample Q. sub-1 demonstrated the most promising antibacterial capacity. According to the results, the acorn shell extracts exhibited promising potential, and it may be interesting to conduct a deeper study on the samples of this species.