Browsing by Author "Andrade, María J."
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- Editorial: Microbial safety of animal-based food productsPublication . Andrade, María J.; Álvarez, Micaela; Rodrigues, PaulaThe studies gathered in this Research Topic focus on several key areas to ensure the safety of animal food products. Firstly, rapid pathogen detection is being improved through technologies like SWIR-HSI combined with advanced machine-learning techniques. Secondly, the use of natural antimicrobials, such as essential oils delivered via liposomes, is being explored to combat microbial development while minimizing negative impacts on food quality. Thirdly, investigations have revealed a link between high crayfish consumption and rhabdomyolysis, emphasizing the need to control the toxins in the food chain. Finally, studies on the relationship between diet and colorectal cancer have suggested a potential inverse association between high fish intake and risk of developing colorectal adenoma, suggesting a possible preventative role for fish consumption. These combined efforts aim to enhance the safety and quality of animal-derived foods through advanced detection methods, natural preservation strategies, and a broader understanding of foodborne threats.
- Proteomic evaluation of the effect of antifungal agents on Aspergillus westerdijkiae ochratoxin A production in a dry-cured fermented sausage-based mediumPublication . Álvarez Rubio, Micaela; Núñez, Félix; Delgado, Josué; Andrade, María J.; Rodrigues, PaulaAspergillus westerdijkiae may produce large amounts of ochratoxin A (OTA) in dry-cured meat products. Natural strategies to control ochratoxigenic moulds using biocontrol agents (BCAs) are currently in the spotlight. The aim of this study was to test the effects of Debaryomyces hansenii and its combination with rosemary derivatives and with a commercial antifungal preparation composed by natamycin and potassium sorbate (AP) against A. westerdijkiae in a dry-cured fermented sausage based-medium. The yeast and rosemary leaves were added to the medium, and rosemary essential oil and AP were added on the casings put on the medium surface to simulate the real product. The growth rate, OTA production and comparative proteomics were analysed. The mould growth in the presence of each treatment was not indicative of their efficiency on OTA repression. The treatment with AP did not affect to the OTA concentration, maybe as consequence of the stressful stimulation of the subinhibitory doses used. D. hansenii added alone or with rosemary showed the best results, decreasing the OTA production >80 %, suggesting that it can be useful as preservative agent during industrial processing. Attending to the proteomic results, its antifungal activity seems to be based on the reduction in abundance of proteins involved in OTA biosynthesis and in the cell wall integrity pathway.
- Rosmarinus officinalis reduces the ochratoxin A production by Aspergillus westerdijkiae in a dry-cured fermented sausage-based mediumPublication . Álvarez Rubio, Micaela; Andrade, María J.; Delgado, Josué; Núñez, Félix; Román, Ángel-Carlos; Rodrigues, PaulaAspergillus westerdijkiae is an ochratoxin A (OTA) producer mould in dry-cured meat products. To prevent the presence of ochratoxigenic moulds in dry-cured fermented sausages, natural strategies based on the use of herbs and their derivatives are in the spotlight. The aim of this study was to test the potential antiochratoxigenic effects of rosemary leaves (R) and rosemary essential oil (REO) as biocontrol agents (BCAs) in a dry-cured fermented sausage-based medium. The mechanisms involved in their effects were also analysed by Proteomics. A control without BCAs and three treatments (R, REO and the combination of R + REO) were carried out. No significant differences were detected when BCAs were individually applied, whilst a significant reduction up to 73.87% in OTA was provoked by R + REO. This combination showed a synergistic effect, in which proteins from PKS ER domain and cell wall integrity pathway seem to be involved. REO alone exerted similar effect on the mould proteome than R + REO, but in a lesser extent. The impact of R in this synergy has not been completely elucidated. The combination of R + REO together with other strategies could minimise the hazard posed by A. westerdijkiae in dry-cured fermented sausages.