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Title: Lipase assay in yarrowia lipolytica
Authors: Morín, M.
Gonzalez, Francisco J.
Choupina, Altino
Burguillo, Francisco J.
Dominguez, Ángel
Issue Date: 2003
Publisher: Springer Verlag
Citation: Choupina, Altino; González, Francisco; Morin, Matias (2003) - Lipase assay in yarrowia lipolytica. In Chapter 60 de Wolf, K.; Breunig, K.; Barth, G. (eds.) Non-conventional yeasts in genetics, biochemistry and, biotechnology: pratical protocols Berlin: Springer-Verlag. p. 373-377. ISBN 3540442154
Abstract: Lipases (triacylglycerol acyl hydrolases, EC 3. 1.1.3) are key enzymes in fat metabolism produced by microorganisms, plants, and animals that catalyze the breakdown of triacylglycerols to free fatty adds and glycerol. In Y. lipolytica severaJ genes encoding lipases have been isoiated,LlP2 coding for a secreted lipase (Pignede et al. 1000) and LlPl and LIP3 encoding two lipase genes of the carboxylesrerase family (Choupina et al. 1999). Lipase expression was repressed by glucose and induced by fatty acids and olive oil.
Peer review: yes
ISSN: 3540442154
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