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Title: Characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) varieties with five isozyme systems
Authors: Morales Corts, Remedios
Rodrigues, Luciano
Ortiz, Jesús María
Pérez Sánches, Rodrigo
Keywords: dendrogram; polymorphism; starch gel electrophoresis; zymogram.
Issue Date: 2008
Publisher: Sociedade Brasileira de Fruticultura
Citation: Corts, Remedios Morales; Rodrigues, Luciano; Ortiz, Jesús Maria; Sánches Pérez, Rodrigo (2008) - Characterization of sour (Prunus cerasus L.) and sweet cherry (Prunus avium L.) varieties with five isozyme systems. Revista Brasileira de Fruticultura. ISSN 0100-2945. 30:1, p. 154-158
Abstract: Extracts from young leaves of nine sweet cherry (Prunus avium L.) and eight sour cherry (Prunus cerasus L.) varieties, located in the germplasm collection of the 'Direção Regional de Agricultura da Beira Interior' (Fundão, Portugal), were analysed for five isozyme systems in order to characterise these varieties and detect problems of synonymies and homonymies that frequently present. The sweet and sour cherry varieties analyzed showed low isoenzymatic polymorphism, being PGM and PGI the systems with the highest discrimination power. These systems presented seven and five different zymogrames, respectively. IDH showed four patterns. SKDH and 6-PGD grouped the varieties only into two patterns. The evident and discriminant restrictions of this type of analysis had got results that have only been a complement for agronomical and morphological characterization. Nove extratos de folhas com desenvolvimento encompleto foram obtidos de 9 variedades de cerejeira (Prunus avium L.) e 8 de ginjeira (Prunus cerasus L.), localizados no campo de germoplasma da "Direcção Regional de Agricultura da Beira Interior" (Fundão, Portugal). Esses extratos foram analisados para 5 sistemas isoenzimáticos a fim de caracterizar essas variedades e simultaneamente detectar sinonímias e homonímias, freqüentes neste tipo de estudos. As variedades de cerejeira e ginjeira analisadas mostraram baixo polimorfismo isoenzimático, sendo que os sistemas PGM e PGI mostraram maior poder de discriminação. Estes sistemas apresentaram 7 e 5 zimogramas diferentes, respectivamente. IDH mostrou 4 padrões. SKDH e 6–PGD agruparam as variedades em 2 padrões. Os evidentes limites discriminatórios desse tipo de análise serviram somente de complemento para a caracterização agronômica e morfológica.
Peer review: yes
ISSN: 0100-2945
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Appears in Collections:PTV - Artigos em Revistas Indexados ao ISI/Scopus

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