Repository logo
 
Publication

The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway

2023Journal article Open access
Simple item page
dc.contributor.authorZhang, Meng-Qi
dc.contributor.authorSun, Ke-Xin
dc.contributor.authorGuo, Xu
dc.contributor.authorChen, Ying-Ying
dc.contributor.authorFeng, Cai-Yun
dc.contributor.authorChen, Jia-Shu
dc.contributor.authorBarreira, João C.M.
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorSun, Jin-Yue
dc.contributor.authorZhang, Jian-Dong
dc.contributor.authorLi, Ningyang
dc.contributor.authorLiu, Chao
dc.date.accessioned2013-01-03T16:58:24Z
dc.date.available2013-01-03T16:58:24Z
dc.date.issued2023
dc.description.abstractEthnopharmacological relevance: Astragali Radix (AR) is the dry root of the leguminous plants Astragalus membranaceus (Fisch) Beg. var. mongholicus (Beg) Hsiao, and Astragalus membranaceus (Fisch) Bge., being used as a medicinal and edible resource. AR is used in traditional Chinese medicine prescriptions to treat hyperuricemia, but this particular effect is rarely reported, and the associated mechanism of action is still need to be elucidated.Aim of the study: To research the uric acid (UA)-lowering activity and mechanism of AR and the representative compounds through the constructed hyperuricemia mouse and cellular models.Materials and methods: In our study, the chemical profile of AR was analysed by UHPLC-QE-MS, as well as the mechanism of action of AR and the representative compounds on hyperuricemia was studied through the constructed hyperuricemia mouse and cellular models.Results: The main compounds in AR were terpenoids, flavonoids and alkaloids. Mice group treated with the highest AR dosage showed significantly lower (p < 0.0001) serum uric acid (208 & PLUSMN; 9 & mu;mol/L) than the control group (317 & PLUSMN; 11 & mu;mol/L). Furthermore, UA increased in a dose-dependence manner in urine and faeces. Serum creatinine and blood urea nitrogen standards, as well as xanthine oxidase in mice liver, decreased (p < 0.05) in all cases, indicating that AR could relieve acute hyperuricemia. UA reabsorption protein (URAT1 and GLUT9) was down-regulated in AR administration groups, while the secretory protein (ABCG2) was up-regulated, indicating that AR could promote the excretion of UA by regulating UA transporters via PI3K/Akt signalling pathway.Conclusion: This study validated the activity, and revealed the mechanism of AR in reducing UA, which provided experimental and clinical basis for the treatment of hyperuricemia with it.por
dc.description.sponsorshipThis work was supported by the Central Guidance on Local Science and Technology Development Fund of Shandong, China (No. YDZX2022175; YDZX2022087); the Innovation Ability Improvement Project for Technology-Based Small and Medium-sized Enterprises of Shandong, China (No. 2022TSGC2002, 2022TSGC2065); the Provincial Major Scientific and Technological Innovation Project of Shandong, China (No. 2021SFGC0904, 2021TZXD001, 2022TZXD0029, 2022TZXD0032), the Natural Science Foundation of Shandong, China (No. ZR2020QD103; ZR2020MH401; ZR2021QH351).
dc.identifier.citationZhang, Meng-Qi; Sun, Ke-Xin; Guo, Xu; Chen, Ying-Ying; Feng, Cai-Yun; Chen, Jia-Shu; Barreira, João C.M.; Prieto Lage, Miguel A.; Sun, Jin-Yue; Zhang, Jian-Dong; Li, Ningyang; Liu, Chao (2023). The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway. Journal of Ethnopharmacology. eISSN 1872-7573. 317, p. 1-11por
dc.identifier.doi10.1016/j.jep.2023.116770
dc.identifier.eissn1872-7573
dc.identifier.issn0378-8741
dc.identifier.urihttp://hdl.handle.net/10198/7803
dc.language.isoengpor
dc.publisherElsevier
dc.subjectAstragali Radixpor
dc.subjectHyperuricemiapor
dc.subjectUA Transporterpor
dc.subjectMechanism
dc.titleThe antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathwaypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Ethnopharmacologypor
person.familyNameBarreira
person.familyNamePrieto Lage
person.givenNameJoão C.M.
person.givenNameMiguel A.
person.identifier107688
person.identifier.ciencia-id221E-1E00-AB74
person.identifier.orcid0000-0003-1233-0990
person.identifier.orcid0000-0002-3513-0054
person.identifier.ridD-8269-2013
person.identifier.ridG-4516-2011
person.identifier.scopus-author-id54895546900
person.identifier.scopus-author-id35937495700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4629b12c-39b0-4da8-8b8d-6efba5cf2d81
relation.isAuthorOfPublication13260615-fd28-4b8b-9dd4-8a8466007579
relation.isAuthorOfPublication.latestForDiscovery4629b12c-39b0-4da8-8b8d-6efba5cf2d81

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
The antihyperuricemia activity of Astragali Radix.pdf
Size:
8.92 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus