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Quantification of three phenolic classes and total phenolic content of propolis extracts using a single UV-vis spectrum

2017Journal article Open access
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dc.contributor.authorPaula, Vanessa B.
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorDias, L.G.
dc.date.accessioned2020-06-05T09:23:24Z
dc.date.available2020-06-05T09:23:24Z
dc.date.issued2017
dc.description.abstractThis work presents a methodology for simultaneously measuring the total content of three classes of phenolic compounds (hydroxybenzoic acids, hydroxycinamic acids and flavonoids), as well the total phenolic content using a single UVvis spectrum. To test the methodology, samples of propolis were used. Firstly, an experimental design based in the Response Surface Methodology established that the best hydroethanolic solution for propolis phenolic compounds extraction had 80% of ethanol. Secondly, calibration models were developed with multiple linear regression, coupled with the leaps algorithm for variable selection, using UV-vis spectra of mixed standard solutions (gallic acid, ferulic acid and quercetin mixtures), all with orthogonal concentrations established by a multilevel fractional factorial design. The model’s estimation and prediction performance had linearity between predicted and expected concentration values, with slope ranging from 0.996–1.04 and determination coefficients higher than 0.995. A quality control solution presented acceptable repeatability and accuracy (relative standard deviation percentage and percentage relative error were lower than 4.8%). Analysis of propolis phenolic compounds extracts showed acceptable precision (relative standard deviation percentages lower than 1.6%) and acceptable accuracy (recoveries assays ranged between 98 and 112%). Overall, the present multi-parametric analytical technique can be a first approach for chemical characterization of phenolic compounds extracts, such as that obtained from the propolis samples, considering its advantages of simplicity, rapidity, precision and accuracy. Moreover, this methodology can be adapted to other sample matrices, provided that the typical UV-vis absorption spectra of their classes of phenolic compounds shows partial overlapping, allowing multivariate calibration.pt_PT
dc.description.abstractEste trabajo presenta una metodologı´a para medir simulta´neamente el contenido total de tres clases de compuestos feno´ licos (a´cidos hidroxibenzoicos, a´cidos hidroxicina´micos y flavonoides), ası´ como el contenido feno´ lico total usando un solo espectro UV-Vis. Para probar la metodologı´a, se utilizaron muestras de propo´ leos. En primer lugar, un disen˜o experimental basado en la metodologı´a de la respuesta en superficie establecio´ que la mejor solucio´n hidroetano´ lica para la extraccio´n de compuestos feno´ licos de propo´ leos tenı´a un 80% de etanol. En segundo lugar, se desarrollaron modelos de calibracio´n con regresio´n lineal mu´ ltiple, junto con el algoritmo de saltos para la seleccio´n variable, utilizando espectros UV-Vis de soluciones esta´ndar mixtas (a´cido ga´lico, a´cido feru´lico y mezclas de quercetina), todas con concentraciones ortogonales establecidas por un disen˜o factorial fraccional multinivel. La estimacio´n del modelo y el rendimiento de la prediccio´n tenı´an linealidad entre los valores de concentracio´n previstos y esperados, con una pendiente que variaba de 0,996 a 1,04 y coeficientes de determinacio´n superiores a 0,995. Una solucio´n de control de calidad presento´ aceptable repetibilidad y precisio´n (porcentaje de desviacio´n esta´ndar relativa y porcentaje de error relativo inferiores al 4,8%). El ana´lisis de extractos de compuestos feno´ licos de propo´ leos mostro´ una precisio´n aceptable (porcentajes de desviacio´n esta´ndar relativa inferior al 1,6%) y una precisio´n aceptable (los ensayos de recuperacio ´n variaron entre 98 y 112%). En general, la presente te´cnica analı´tica multiparame´trica puede ser un primer enfoque para la caracterizacio´n quı´mica de extractos de compuestos feno´ licos, como el obtenido a partir de las muestras de propo´ leos, considerando sus ventajas de simplicidad, rapidez, precisio´n y precisio´ n. Adema´s, esta metodologı´a puede adaptarse a otras matrices de muestra, siempre que los espectros de absorcio´n UV-Vis tı´picos de sus clases de compuestos feno´ licos muestren solapamiento parcial, permitiendo la calibracio´n multivariante.pt_PT
dc.description.sponsorshipThis work was financially supported by CQ-VR, Centro de Química – Vila Real, University of Trás-os-Montes e Alto Douro [Project UID/QUI/00616/2013]; Centre of Molecular and Environmental Biology, University of Minho [Project UID/ BIA/04050/2013]; funded by FEDER (Fundo Europeu de Desenvolvimento Regional) through COMPETE2020 (Programa Operacional Competitividade e Internacionalização, POCI); by national funds through FCT (Fundação para a Ciência e a Tecnologia, Portugal); by Project 56343–“New products derived from sausage”, Measure 4.1 – Cooperation for Innovation, PRODER program, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPaula, Vanessa B.; Estevinho, Leticia M.; Dias, L.G. (2017). Quantification of three phenolic classes and total phenolic content of propolis extracts using a single UV-vis spectrum. Journal of Apicultural Research. ISSN 0021-8839. 56:5, p. 569-580pt_PT
dc.identifier.doi10.1080/00218839.2017.1370904pt_PT
dc.identifier.issn0021-8839
dc.identifier.urihttp://hdl.handle.net/10198/22026
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectPropolis analysispt_PT
dc.subjectHydroxybenzoic acidspt_PT
dc.subjectHydroxycinamic acidspt_PT
dc.subjectFlavonoidspt_PT
dc.subjectTotal phenolic contentpt_PT
dc.subjectSingle UV-vis spectrumpt_PT
dc.subjectExperimental designpt_PT
dc.subjectMultivariate calibrationpt_PT
dc.titleQuantification of three phenolic classes and total phenolic content of propolis extracts using a single UV-vis spectrumpt_PT
dc.title.alternativeCuantificacio´n de tres clases feno´ licas y contenido feno´ lico total de extractos de propo´ leos utilizando un u´ nico espectro UV-Vispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00616%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F04050%2F2013/PT
oaire.citation.endPage580pt_PT
oaire.citation.startPage569pt_PT
oaire.citation.titleJournal of Apicultural Researchpt_PT
oaire.citation.volume56 (5)pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNamePaula
person.familyNameEstevinho
person.familyNameDias
person.givenNameVanessa B.
person.givenNameLetícia M.
person.givenNameLuís G.
person.identifier.ciencia-id5F16-A866-562D
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.orcid0000-0001-7281-8598
person.identifier.orcid0000-0002-9249-1948
person.identifier.orcid0000-0002-1210-4259
person.identifier.scopus-author-id6506577664
person.identifier.scopus-author-id23569169900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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