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Development of LAMP Primers for the Detection of Pyrethroid Resistance Mutations in Varroa destructor

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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorCosta, Maíra
dc.contributor.authorYadró García, Carlos A.
dc.contributor.authorLopes, Ana
dc.contributor.authorBejaoui, Mohamed Khalil
dc.contributor.authorAlmeida, Jhennifer
dc.contributor.authorCorreia, Lucas
dc.contributor.authorSánchez, Sara
dc.contributor.authorLi, Fernanda
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.date.accessioned2025-06-23T15:56:46Z
dc.date.available2025-06-23T15:56:46Z
dc.date.issued2025
dc.description.abstractVarroa destructor is one of the main threats to Apis mellifera L., directly affecting colony health and contributing to their global decline. Control of this mite is traditionally achieved using acaricides, with pyrethroids such as tau-fuvalinate and fumethrin being the most used, acting on voltage-gated sodium channels (VGSC). However, the intensive use of these compounds by beekeepers has led to the emergence of resistance, associated with mutations at residues 918 and 925 of the VGSC gene [1]. Traditional methods for detecting these mutations, such as PCR, TaqMan and RT-PCR, are e𿿿ective but require expensive laboratory equipment. In this context, Loop-mediated Isothermal Amplification (LAMP) is a promising alternative, offering rapid and cost-effective detection without the need for thermal cycling [2]. LAMP is based on the use of a set of four to six primers, including two inner primers (FIP and BIP), two outer primers (F3 and B3), and optionally two loop primers (LoopF and LoopB), which are introduced to accelerate the amplification reaction [3]. This study aimed to develop specific LAMP primers, using the NEB LAMP software, for the detection of the main mutations associated with Varroa destructor resistance to pyrethroids in Portugal. The predictive efficiency, specificity, and thermodynamic properties of the designed primers were assessed using BLAST, eLAMP, and OligoAnalyzer tools, considering qPCR parameters. This work successfully identified specific primer sets, including loop primers, for the detection of the mutation at position 925, which may be used in future experimental validations for rapid diagnostic applications.por
dc.identifier.citationCosta, Maíra; Yadró García, Carlos A.; Lopes, Ana; Bejaoui, Mohamed Khalil; Almeida, Jhennifer; Correia, Lucas; Sánchez, Sara; Li, Fernanda; Pinto, Maria Alice; Henriques, Dora (2025). Development of LAMP Primers for the Detection of Pyrethroid Resistance Mutations in Varroa destructor. In International Conference of the Portuguese Society of Genetics. Oeiras
dc.identifier.urihttp://hdl.handle.net/10198/34617
dc.language.isoeng
dc.peerreviewedno
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLAMP Primers
dc.titleDevelopment of LAMP Primers for the Detection of Pyrethroid Resistance Mutations in Varroa destructoreng
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferenceDate2025-06
oaire.citation.conferencePlaceOeiras
oaire.citation.endPage69
oaire.citation.startPage69
oaire.citation.titleInternational Conference of the Portuguese Society of Genetics
oaire.versionhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43
person.familyNameCosta
person.familyNameYadró García
person.familyNameLopes
person.familyNamePinto
person.familyNameHenriques
person.givenNameMaíra
person.givenNameCarlos A.
person.givenNameAna
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
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relation.isAuthorOfPublication.latestForDiscoveryc7b97b29-3139-43ff-ae85-23f613e280ed

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