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Evaluation of virulence and the oxalic acid production on Cryphonectria parasitica virulent and converted strains by CHV1 hypovirus

2021Conference object Open access
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dc.contributor.authorGadoum, Horiya
dc.contributor.authorCoelho, Valentim
dc.contributor.authorNoui, Abdallah
dc.contributor.authorJorge, Lurdes
dc.contributor.authorGouveia, Maria Eugénia
dc.date.accessioned2021-07-21T09:23:15Z
dc.date.available2021-07-21T09:23:15Z
dc.date.issued2021
dc.description.abstractChestnut Blight, caused by Cryphonectria parasitica (Murrill) Bar, is a major disease in Castanea sativa Mill. on the European continent. Biological control by hypovirulence is a sustainable and efficient method to control the disease. The presence of Cryphonectria hypovirus 1 (CHV1) in C. parasitica reduces the fungus virulence that promote canker healing and tree recovery. Hypovirus infection results in phenotypic and metabolic changes, including the reduction of ligninolytic enzymes activity, and decreased oxalic acid production. The aim of this work was to evaluate the oxalic acid production in both virulent and converted strains on PDB (Potato Dextrose Broth, 24g/L) and to access the virulence of these strains on chestnut stems. Six isolates were converted with two characterized hypovirulent C. parasitica isolates (RBB111, SR44.2) and the presence of CHV1 was detected by molecular methods. Oxalic acid production was evaluated by spectrophotometry after the growth of the strains on 100 ml of PDB supplemented with 2mM MnSO4 in an orbital incubator during five days. To evaluate the virulence of the isolates, chestnut stems were inoculated with the virulent isolates, their converted ones and the hypovirulent isolates. The characterized hypovirulent isolates used in this work has complete ability to convert virulent isolates with effective hypovirus transmission and PCR detection of CHV1 was obtained in all C. parasitica converted strains. The obtained results by spectrophotometric analysis have revealed that virulent strains always produced more oxalic acid than converted strains. The infection area on chestnut stems caused by virulent strains was significantly higher (P<0.05) than the infection area caused by converted strains. The converted strains Cast26/RBB111 and Cast26/SR44.2 showed 50% and 88.6% reduction in the content of oxalic acid present in supernatant, respectively. This suggests that the reduction in enzymatic activities caused by hypovirulent strains is variable with the hypovirulent donor used in conversion.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGadoum, Horiya; Coelho, Valentim; Noui, Abdallah; Jorge, Lurdes; Gouveia, Maria Eugénia (2021). Evaluation of virulence and the oxalic acid production on Cryphonectria parasitica virulent and converted strains by CHV1 hypovirus. In 3rd International Conference on Food, Agriculture and Veterinary. Izmir. ISBN 978-625-7720-43-4pt_PT
dc.identifier.isbn978-625-7720-43-4
dc.identifier.urihttp://hdl.handle.net/10198/23749
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEuropean chestnutpt_PT
dc.subjectPathogenesispt_PT
dc.subjectOxalatept_PT
dc.titleEvaluation of virulence and the oxalic acid production on Cryphonectria parasitica virulent and converted strains by CHV1 hypoviruspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceIzmir-TURKEYpt_PT
oaire.citation.endPage122pt_PT
oaire.citation.startPage121pt_PT
oaire.citation.title3rd INTERNATIONAL CONFERENCE on FOOD, AGRICULTURE and VETERINARYpt_PT
person.familyNameCoelho
person.familyNameGouveia
person.givenNameValentim
person.givenNameMaria Eugénia
person.identifier.ciencia-id0618-49BC-EACE
person.identifier.orcid0000-0001-5829-7669
person.identifier.orcid0000-0002-2550-9108
person.identifier.scopus-author-id26533798200
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication99a97d58-8b4c-4a49-9855-d334d568b9f3
relation.isAuthorOfPublication99e4701b-e3c2-4594-a37f-42bd49821d6e
relation.isAuthorOfPublication.latestForDiscovery99a97d58-8b4c-4a49-9855-d334d568b9f3

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