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Advisor(s)
Abstract(s)
Bee-pollen is a food produced by bees from the flower pollen, to be a source of
proteins, lipids, vitamins and minerals for the hive [1]. Its composition varies according
to the region where it is produced, and its quality is influenced by the collection and
processing conditions [2, 3]. Besides nutritional substances, bee-pollen contains
significant amounts of polyphenols with recognized health benefits, including
antioxidant activity [4].
The present study aimed to evaluate the polyphenolic profile and antioxidant
activity of 56 dehydrated bee-pollen samples by high performance liquid
chromatography and in vitro assays. Bee-pollens samples, collected during November
2011 to December 2013 from apiaries located in different Brazilian regions, were
extracted with stirring for 30 minutes with ethanol 70% at 70 ºC and analyzed. The
total phenolic content ranged from 6.5 ± 0.2 to 29.2 ± 0.3 mg gallic acid equivalent/g
dry sample and flavonoid content ranged from 0.35 ± 0.01 to 17.5 ± 0.1 mg quercetin
equivalent/g dry sample. The antioxidant activity assays, with values of 9.4 ± 0.4 to
155 ± 5 mol Trolox equivalent/g dry sample for DPPH and 133 ± 2 to 563 ± 15 mol
TE/g for ORAC methods, revealed the antioxidant capacity of bee-pollen extracts.
Regarding, the characterization of the phenolic composition from the several extracts
by HPLC-PAD it was possible to observe that rutin, quercetin and vanillic acid were the main phenolic compounds found in the Brazilian dehydrated bee-pollen analyzed.
On the other hand, caffeic acid was found in less amounts in the majority of samples
studied. Among the identified polyphenols, only quercetin seems to have influenced
positively in the antioxidant capacity of the samples. The Pearson’s Correlation
analysis indicated high correlation between quercetin and ORAC values (r=0.6570,
p=0.000) and medium correlation between quercetin and DPPH values (r=0.4873,
p=0.000). In conclusion, bee-pollen characteristics vary according to the botanical
origin and the Brazilian region where the sample was produced. This is a product with
high antioxidant potential, therefore other tests, especially bioavailability assays,
should be performed.
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Citation
Melo, Adriana A.; Estevinho, Leticia M.; Moreira, Manuela; Delerue-Matos, Cristina; Almeida-Muradian, Ligia (2015). Phenolic profile and antioxidant capacity from 56 brazilian dehydrated bee-pollen. In XVI Latin-American Congress on Chromatography 9th National Meeting on Chromatography. Lisboa