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Chemical and bioactive characterization of Melissa officinalis L. subjected to sustainable cultivation: comparison between different extraction methods

2021Conference object Open access
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dc.contributor.authorOliveira, Izamara
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorCarocho, Márcio
dc.contributor.authorDias, Maria Inês
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorPetropoulos, Spyridon Α.
dc.contributor.authorTzortzakis, Nikolaos
dc.contributor.authorChrysargyris, Antonios
dc.contributor.authorMandim, Filipa
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-02-01T16:21:49Z
dc.date.available2022-02-01T16:21:49Z
dc.date.issued2021
dc.description.abstractMelissa officinalis L. (lemon balm) is a species of the Lamiaceae family which has been consumed for several decades in the form of decoctions, infusions or directly in food preparations. In addition to its recognized effects in helping digestion problems, rheumatism or headaches, several studies have demonstrated its antioxidant, hypoglycemic, hypolipidemic, antimicrobial, anticancer, antidepressant, anxiolytic, anti-inflammatory, and spasmolytic capacities. In this study it was aimed to compare three different extraction methods: infusion (100% water), maceration (80:20 ethanol: water) and ultrasound assisted extraction (UAE) under previous optimized extraction conditions (33.0 ± 3.2 min, 371.7 ± 19.3 W and 39.9 ± 1.4% ethanol) in plants grown under sustainable cultivation on complete irrigation [1]. The studied parameters included the bioactive evaluation through antimicrobial (microdilution method), antioxidant (thiobarbituric acid reactive substances - TBARS), cytotoxicity (sulforhodamine B) and antiinflammatory (RAW cells) assays. It was also studied the phenolic compounds and organic acids composition. According to the obtained results, eight phenolic compounds were identified and quantified, being rosmarinic acid the major one (107.1 ± 0.9 mg/g of extract). Except for lithospermic acid A isomer II (2.38 ± 0.03 mg/g of extract) and hydoxysalvioanolic acid E (20.7 ± 0.1 mg/g of extract), the infusion revealed the lowest extraction of individual polyphenols, while the maceration recorded the highest extractability. On the other hand, the content of six out of the eight detected polyphenols for the ultrasound-assisted extraction was between the infusion and maceration methods. In terms of antioxidant activity determined through the TBARS assay, the infusions showed the highest activity (1.47 ± 0.05 μg/mL), followed by UAE (2.14 ± 0.03 μg/mL) and maceration (5.79 ± 0.06 μg/mL). The antiinflammatory activity showed the opposite trend, with the maceration showing the strongest potential (114 ± 6 μg/mL), followed by infusion (292 ± 6 μg/mL) and UAE (316 ± 8 μg/mL). The antitumor properties were assessed in 5 cell lines, with the best results being recorded for maceration, except in CaCo2 cells, where infusion showed the best results (48 ± 1 μg/mL). All in all, these natural extracts are interesting ingredients, capable of replacing counterparts of synthetic origin, and may find wide applications in the industrial sector (e.g., food, pharmaceutical and cosmetic companies). Besides, a great potential of using the leaves of M. officinalis to obtain extracts enriched in rosmarinic acid was presented, which may be included in developmental strategies for increased plant production.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). L. Barros and M.I. Dias thank the national funding by FCT through the institutional scientific employment program-contract for her contract, while M. Carocho and S. Heleno thank FCT through the individual scientific employment program-contracts (CEECIND/00831/2018 and CEECIND/03040/2017). I. Oliveira thanks FCT for her PhD grant (BD/06017/2020). To FEDER-Interreg España-Portugal programme for financial support through the project TRANSCoLAB 0612_TRANS_CO_LAB_2_P; to ERDF through the Regional Operational Program North 2020, within the scope of Project GreenHealth - Norte-01-0145-FEDER-000042.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOliveira, Izamara de; Heleno, Sandrina; Carocho, Márcio; Dias, Maria Inês; Santos-Buelga, Celestino; Petropoulos, Spyridon; Tzortzakis, Nikolaos; Chrysargyris, Antonios; Mandim, Ana Filipa; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Chemical and bioactive characterization of Melissa officinalis L. subjected to sustainable cultivation: comparison between different extraction methods. In 5th International Symposium on Phytochemicals in Medicine and Food. Nanchang, Chinapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/24995
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationBD/06017/2020pt_PT
dc.relationMountain Research Center
dc.relationNot Available
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMelissa officinalis L.
dc.subjectLemon balm
dc.titleChemical and bioactive characterization of Melissa officinalis L. subjected to sustainable cultivation: comparison between different extraction methodspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleNot Available
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F01011%2F2018%2FCP1578%2FCT0002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03040%2F2017%2FCP1403%2FCT0001/PT
oaire.citation.title5th International Symposium on Phytochemicals in Medicine and Food (5-ISPMF)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2018
oaire.fundingStreamCEEC IND 2017
person.familyNameOliveira
person.familyNameHeleno
person.familyNameCarocho
person.familyNameDias
person.familyNameMandim
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person.familyNameBarros
person.givenNameIzamara
person.givenNameSandrina A.
person.givenNameMárcio
person.givenNameMaria Inês
person.givenNameFilipa
person.givenNameIsabel C.F.R.
person.givenNameLillian
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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