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Extraction of bioactive compounds from plants as promising agentes against SARS-CoV-2

2021Conference object Open access
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dc.contributor.authorPedrosa, Mariana C.
dc.contributor.authorRodrigues, Cláudia
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorCarocho, Márcio
dc.contributor.authorAbreu, Rui M.V.
dc.contributor.authorRibeiro, Tânia
dc.contributor.authorMachado, Manuela
dc.contributor.authorPinto, Manuel M.
dc.contributor.authorSimas, João P.
dc.contributor.authorPintado, Manuela
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-02-22T10:48:21Z
dc.date.available2022-02-22T10:48:21Z
dc.date.issued2021
dc.description.abstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pathogenic virus with high transmissibility and infectivity, which began to spread across the globe in late 2019, which soon became the COVID-19 pandemic, causing social and economic impacts. In response to this situation, the scientific community started the development of effective substances against this virus. Bioactive molecules present in plants, mainly phenolic compounds, are promising altematives to combat pathological disorders. Therefore, the objective of this work was to use the aqueous extract of a mountain plant as an antiviral substance to neutralize COVID-19. MaterialslMethods: The mountain plant extract was obtained by dynamic maceration in water for I hour (twice). Afier obtaining the extracts, they were evaluated for their phenolic profile by high performance líquid chromatography coupIed to a diode array detector and a mass spectrometer detector (HPLC-DAD-MS). Cytotoxicíty was determined by the sulphorhodamine B assay in Vero cells, as well as the evaluation ofthe antivíral activity. Results: Regarding the phenolic profile, the main compounds found were trigalloyl-HHDPglucoside; pentagalloyl glucose, quercetin 3-0-glucuronide and quercetin O-hexoside. The GI50 (concentration able to inhibit 50% of cell proliferation) and the MNCC (maximum concentration without toxicity) were between 100 and 180.3 J.lg/rnL and between 85 and 120 J.lg/rnL, respectively. The MNCC value was obtained considering the concentration that allowed 90% of celI proliferation of Vero cells. In relation to the viral activity screening, the results achieved for the viral titre were between 5000 and 9000 PFU/rnL, while for the antiviral activity ranged between 0.5 and 3.0 Mv, being the percentage ofreduction in a range of85-95 %. • Conclusion: The mountain plant extracts showed in its composition bioactive compounds and consistent results of antiviral activity. Moreover, it presents itself as a potential substance for protection applications against the COVID-19 virus. However, further studies in specific products are required for validation and implementation.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FeT, Portugal) for financiai support tbrough national funds FCT/MCTES to the CIMO (UIDB/00690/2020). M.C. Pedrosa tbanks "PlantCovid" projeci for her scholarship. S. Helena and M. Carocho fhank FCT for their individual emplayment program·contract (CEECTND/00831/20J8, CEECINDI03040/2017). L. Barros also thanks the national funding by FCr , through the institutional scientific employment program--contract for her contract. Acknowledgments to the Project financed by the European Fund for Regional Development (Fundo Europeu de Desenvolvimento Regional (FEDER)) through lhe Programa Operacional Regional Norte 2020, within the "PlantCovid" project, NORTE-01-02B7-FEDER-054870pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPedrosa, Mariana C.; Rodrigues, Cláudia; Heleno, Sandrina A.; Carocho, Márcio; Abreu, Rui M.V.; Ribeiro, Tânia; Machado, Manuela; Pinto, Manuel M.; Simas, João P.; Pintado, Manuela; Barros, Lillian (2021). Extraction of bioactive compounds from plants as promising agentes against SARS-CoV-2. In 4th International Congress of Bíochemistry and Microbiology Applied Technologie: book of abstracts. Hammametpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/25101
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNORTE-01-02B7-FEDER-054870pt_PT
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioactive compoundspt_PT
dc.titleExtraction of bioactive compounds from plants as promising agentes against SARS-CoV-2pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceHammametpt_PT
oaire.citation.endPage144pt_PT
oaire.citation.startPage144pt_PT
oaire.citation.title4th International Congress of Bíochemistry and Microbiology Applied Technologie: book of abstractspt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePedrosa
person.familyNameHeleno
person.familyNameCarocho
person.familyNameAbreu
person.familyNameBarros
person.givenNameMariana C.
person.givenNameSandrina A.
person.givenNameMárcio
person.givenNameRui M.V.
person.givenNameLillian
person.identifierawbWiiMAAAAJ&hl
person.identifier469085
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person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0002-2057-0639
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-8978-4547
person.identifier.orcid0000-0002-7745-8015
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridL-5951-2014
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id52463229800
person.identifier.scopus-author-id7003290613
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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