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Understanding the transition from embryogenesis to seed filling in Phaseolus vulgaris L. non-endospermic seeds

2025Journal article Open access
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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg15:Proteger a Vida Terrestre
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorLopes, Cláudia
dc.contributor.authorFevereiro, Pedro
dc.contributor.authorAraújo, Susana
dc.contributor.authorAraújo, Susana de Sousa
dc.date.accessioned2025-11-21T14:28:40Z
dc.date.available2025-11-21T14:28:40Z
dc.date.issued2025
dc.description.abstractCommon bean (Phaseolus vulgaris L.) is one of the most consumed grain legumes. These legumes are a major source of proteins and other important nutrients, especially in developing countries. Studying seed development in common bean is crucial for improving yield, nutrition, stress tolerance and disease resistance while promoting sustainable agriculture and food security, with its sequenced genome and available molecular tools making it an excellent research model. Despite advances in studying P. vulgaris seed development, the precise timing and molecular regulation of the transition from embryogenesis to seed filling remain poorly understood. Although P. vulgaris seeds at 10 days after anthesis (DAA) were previously characterized as being in the late embryogenesis stage, our previous studies suggested that this transition might occur earlier than 10 DAA, prompting us to investigate earlier developmental stages.Methods To accomplish this goal, we conducted a comprehensive analysis at 6, 10, 14, 18 and 20 DAA, integrating morphological, histological, and transcriptomic approaches.Results and discussion Morphological and histochemical data revealed that by 10 DAA, cotyledons are fully formed, but storage compound accumulation is only noticed at 14 DAA, indicating that the transition from embryogenesis to seed filling occurs between 10 and 14 DAA. Transcriptomic analysis further supported this finding, showing upregulation of genes associated with seed storage proteins, starch metabolism, and hormonal regulation at 14 and 18 DAA. This study redefines the developmental timeline of P. vulgaris seed filling initiation, bridging a critical knowledge gap in legume seed biology. Given the limited availability of histological studies on early P. vulgaris seed development, our findings provide essential insights into the structural and molecular events driving this transition. By refining the timing and regulatory mechanisms of early seed development, this study lays the groundwork for future research aimed at enhancing seed quality and resilience in legumes.eng
dc.description.sponsorshipThe author(s) declare that financial support was received for the research and/or publication of this article. The authors acknowledgethe financial support from Fundação para a Ciência e a Tecnologia (Portugal) through the R&D Unit “GREEN-IT - Bioresources for Sustainability” (UIDB/04551/2020, DOI: 10.54499/UIDB/04551/2020 and UIDP/04551/2020, DOI: 10.54499/UIDP/04551/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020, DOI: 10.54499/LA/P/0087/2020) and Plants for Life PhD grant of Cláudia Lopes (PD/BD/135585/2018). Author SSA acknowledges financial support from PRR - Investimento RE-C05-i02: Missão Interface - CoLAB and national funds through FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020) and UIDP/00690/2020 (DOI: 0.54499/UIDP/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020).
dc.identifier.citationLopes, Claúdia; Fevereiro, Pedro; Araújo, Susana (2025) Understanding the transition from embryogenesis to seed filling in Phaseolus vulgaris L. non-endospermic seeds. Frontiers In Plant Science. ISNN 1664-462X. 16, p.1-17.
dc.identifier.doi10.3389/fpls.2025.1597915
dc.identifier.issn1664-462X
dc.identifier.urihttp://hdl.handle.net/10198/35125
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media SA
dc.relationGREEN-IT "Bioresources for Sustainability"
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relation.ispartofFrontiers in Plant Science
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/
dc.subjectEarly seed filling
dc.subjectPhaseolus vulgaris L.
dc.subjectSeed histology
dc.subjectStorage compounds
dc.subjectTranscriptome
dc.titleUnderstanding the transition from embryogenesis to seed filling in Phaseolus vulgaris L. non-endospermic seeds
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleGREEN-IT "Bioresources for Sustainability"
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04551%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage17
oaire.citation.startPage1
oaire.citation.titleFrontiers In Plant Science
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAraújo
person.givenNameSusana de Sousa
person.identifier.ciencia-id7015-2AB3-4378
person.identifier.gsidhttps://scholar.google.com/citations?user=jbffwfsAAAAJ&hl=pt-PT&authuser=1
person.identifier.orcid0000-0003-2823-088X
person.identifier.ridA-5482-2009
person.identifier.scopus-author-id9636203600
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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