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Nosema distribution across Portugal. Results from the first nationwide survey (2011-12)

dc.contributor.authorPires, Sancia
dc.contributor.authorMurilhas, António
dc.contributor.authorRusso, Paulo
dc.contributor.authorValério, Maria José
dc.contributor.authorGonçalves, Manuel
dc.date.accessioned2014-01-27T15:30:27Z
dc.date.available2014-01-27T15:30:27Z
dc.date.issued2013
dc.description.abstractA nationwide field sampling exercise was launched in 2011, to address mounting anecdotal evidence of atypically high honey bee colony mortalities of unexplained origin occurring throughout Portugal. The first approach was to contact 662 beekeepers (≈ 4% of the registered Portuguese beekeepers), via telephone interviews with a view to formulating an 'educated guess' regarding the cases where Nosema apis / Nosema ceranae seemed more likely to have had a role in colony mortality/morbidity. Interviewees were selected accounting for their total numbers of colonies and the geographical distribution of their apiaries across the country. The 'interviewee grid' was set to 5 beekeepers per county, fully covering continental Portugal. Following these interviews, a total of 227 apiaries (≈ 3 sampling sites per county), representing the whole continental part of the country, were sampled according to standard methodology (pooled samples of 60 foragers from the outside the hive entrance). All samples were assessed both by standard light microscopy (in a first approach to Nosema spore presence/absence) and by molecular PCR-based methodology (mainly aiming at Nosema spp. identification), as recently published in the 'BEEBOOK'. Nosema ceranae was identified in 51% of the studied apiaries across the country, ranging from 19% (in the southern 'Faro' district) up to 89% (in the northern 'Aveiro' district). Despite the fact that considerable district variation in Nosema ceranae prevalence levels were found throughout mainland Portugal, no biologically meaningful geographical pattern (either associated with north/south, inland/coastal or altitudinal transects) was observed. Nosema apis infected samples were not encountered in this study.por
dc.description.sponsorshipPrograma Apícola Nacional (PAN) - Triénio 2011-2013por
dc.identifier.citationPires, S.; Murilhas, A.M.; Almeida, P.R.; Valério, M.J.; Gonçalves, M. (2013). Nosema distribution across Portugal. Results from the first nationwide survey (2011-12). In 43th International Apicultural Congress. Kiev, Ucrâniapor
dc.identifier.urihttp://hdl.handle.net/10198/9179
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherApimondiapor
dc.relationPAN - Portugal, Nosema e Apiculturapor
dc.subjectNosemapor
dc.subjectBeekeepingpor
dc.subjectPortugalpor
dc.titleNosema distribution across Portugal. Results from the first nationwide survey (2011-12)por
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceKyiv, Ukrainepor
oaire.citation.titleXXXXIII International Apicultural Congresspor
person.familyNamePires
person.givenNameSancia
person.identifier.ciencia-idCC12-1B38-26C8
person.identifier.orcid0000-0001-8427-0179
person.identifier.scopus-author-id53878422600
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationd1319f5d-6ffc-4e2c-9035-fa06b834c88f
relation.isAuthorOfPublication.latestForDiscoveryd1319f5d-6ffc-4e2c-9035-fa06b834c88f

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