Development of an Electrochemical Aptasensor for the Detection of Human Osteopontin

Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R.

http://hdl.handle.net/10198/15823

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Abstract(s)

Electrochemical RNA aptasensor To obtain bio-sensor with high affinity for rhOPN aiming to be used for cancer prognosis Materials and methods RNA aptamer details Isolated by SELEX process [1] Sequence of the biotinylated RNA aptamer 5’-Biotin- CGGCCACAGAAUGAAAAACCUCAUCGAUGUUGCAUAGUUG-3’ Removal the interference of Rnase Working RNA aptamer solution 4 nM in PBS. RNA aptamer immobilization on a gold surfasse via streptavidin-biotin interaction

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http://hdl.handle.net/10198/15823

Citation

Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2014). Development of an Electrochemical Aptasensor for the Detection of Human Osteopontin. In Eurosensors 2014 - The 28th European Conference on Solid-State Transducres. Brecia, Itália