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A simple microfluidic device for the deformability assessment of blood cells in a continuous flow

2015Journal article Open access
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dc.contributor.authorRodrigues, Raquel Oliveira
dc.contributor.authorPinho, Diana
dc.contributor.authorFaustino, Vera
dc.contributor.authorLima, Rui A.
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-01T16:04:44Z
dc.date.accessioned2018-03-05T09:18:59Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-01T16:04:44Z
dc.date.available2018-03-05T09:18:59Z
dc.date.issued2015
dc.description.abstractBlood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability.en_EN
dc.description.sponsorshipThe authors acknowledge the financial support provided by PTDC/SAU-ENB/116929/2010 and EXPL/EMS-SIS/ 2215/2013 from FCT (Fundação para a Ciência e a Tecnologia), COMPETE, QREN and European Union (FEDER). R. O. Rodrigues, D. Pinho and V. Faustino acknowledge respectively, the PhD scholarships SFRH/BD/97658/2013, SFRH/BD/89077/2012 and SFRH/BD/99696/ 2014 granted by FCT. The authors would also like to thank Dr. Ângela Fernandes for providing the blood samples and Dr. Ricardo Calhelha for supplying the tissue culture medium used in this work.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationRodrigues, Raquel O.; Pinho, Diana; Faustino, Vera; Lima, Rui (2015). A simple microfluidic device for the deformability assessment of blood cells in a continuous flow. Biomedical Microdevices. ISSN 1387-2176. 17:108, p. 1-9en_EN
dc.identifier.doi10.1007/s10544-015-0014-2en_EN
dc.identifier.issn1387-2176
dc.identifier.urihttp://hdl.handle.net/10198/16100
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
dc.relationMAGNETIC CARBON NANOSTRUCTURES AND STUDY OF THEIR TRANSPORT IN MICROFLUIDIC DEVICES FOR HYPERTHERMIA
dc.relationMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
dc.subjectBlood on chipsen_EN
dc.subjectCell separation and deformabilityen_EN
dc.subjectHyperbolic microchannelen_EN
dc.subjectMicrofluidic devicesen_EN
dc.subjectRBCen_EN
dc.subjectWBCen_EN
dc.titleA simple microfluidic device for the deformability assessment of blood cells in a continuous flowen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
oaire.awardTitleMAGNETIC CARBON NANOSTRUCTURES AND STUDY OF THEIR TRANSPORT IN MICROFLUIDIC DEVICES FOR HYPERTHERMIA
oaire.awardTitleMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FEMS-SIS%2F2215%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97658%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F99696%2F2014/PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamCOMPETE
oaire.fundingStreamSFRH
oaire.fundingStreamFARH
person.familyNamePinho
person.familyNameLima
person.givenNameDiana
person.givenNameRui A.
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.ciencia-idEE12-C3FB-349D
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0003-3428-637X
person.identifier.ridH-5157-2016
person.identifier.scopus-author-id18437397800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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