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Nanodispersions of beta-carotene: effects on antioxidant enzymes and cytotoxic properties

2018Journal article Open access
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dc.contributor.authorRocha, Felipe
dc.contributor.authorSugahara, Letícia Yumi
dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorOliveira, Sara Marchesan de
dc.contributor.authorBrum, Evelyne da Silva
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorIneu, Rafael P.
dc.contributor.authorGonçalves, Odinei Hess
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-10-29T09:14:02Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-10-29T09:14:02Z
dc.date.issued2018
dc.description.abstractBeta-carotene is a carotenoid precursor of vitamin A, known for its biological activities. Due to its high hydrophobicity, nanonization processes, i.e. the transformation into nanoparticles, can improve its water affinity, and therefore the activity in aqueous systems. The objective of this study was to produce beta-carotene nanoparticles by the solid dispersion method and to evaluate their effects on the activity of glutathione-S-transferase and acetylcholinesterase enzymes using Drosophila melanogaster (DM) homogenate, the superoxide dismutase- and catalase-like activities under in vitro conditions, and their cytotoxic properties against tumor and non-tumor cells. The formed nanometric beta-carotene particles resulted in stable colloids, readily dispersed in water, able to modulate acetylcholinesterase (AChE) activity, and presenting high potential to control the cholinergic system. Beta-carotene nanoparticles, at concentrations much lower than the pure pristine beta-carotene, presented in vitro mimetic activity to superoxide dismutase and altered glutathione-S-transferase activity in DM tissue. The content of hydrogen peroxide was neither affected by the nanoparticles (in aqueous solution) nor by pristine beta-carotene (in DMSO). In the cytotoxic assays, beta-carotene nanoparticles dispersed in water showed activity against four different tumor cell lines. Overall, beta-carotene nanoparticles presented significant bioactivity in aqueous medium surpassing their high hydrophobicity constraint.
dc.description.sponsorshipThe authors thank CNPq, CAPES and Fundação Araucária for the support. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (strategic project UID/AGR/00690/2013) and R. Calhelha contract, and to the project POCI-01-0145- FEDER-006984 – Associate Laboratory LSRE-LCM funded by the FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT. This work was also funded by the European Structural and Investment Funds (FEEI) through the Regional Operational Program North 2020, within the scope of Project NORTE-01-0145-FEDER-023289: DeCodE and Project Mobilizador ValorNatural®.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationRocha, Felipe; Yumi Sugahara, Letícia; Leimann, Fernanda Vitória; De Oliveira, Sara Marchesan; Da Silva Brum, Evelyne; Calhelha, Ricardo Costa; Barreiro, Maria Filomena; Ferreira, Isabel C.F.R.; Porto Ineu, Rafael; Gonçalves, Odinei Hess (2018). Nanodispersions of beta-carotene: effects on antioxidant enzymes and cytotoxic properties. Food and Function. ISSN 2042-6496. 9, p. 3698-3706en_EN
dc.identifier.doi10.1039/c8fo00804cen_EN
dc.identifier.issn2042-6496
dc.identifier.urihttp://hdl.handle.net/10198/18111
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.titleNanodispersions of beta-carotene: effects on antioxidant enzymes and cytotoxic propertiesen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameLeimann
person.familyNameCalhelha
person.familyNameBarreiro
person.familyNameFerreira
person.familyNameGonçalves
person.givenNameFernanda Vitória
person.givenNameRicardo C.
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.givenNameOdinei Hess
person.identifier144781
person.identifier.ciencia-id8F10-A04D-FC0D
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id3418-47D5-5746
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-6230-9597
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9528-8187
person.identifier.ridJ-2172-2014
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id9335958400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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