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Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicator

2025Journal article Open access
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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.fosCiências Médicas::Biotecnologia Médica
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg04:Educação de Qualidade
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorSantos, Dércia
dc.contributor.authorPinto, Ana Rita
dc.contributor.authorBarata, Rita
dc.contributor.authorFernandes, Conceição
dc.contributor.authorGuedes, Hugo
dc.contributor.authorAlmeida, Gonçalo
dc.contributor.authorCabecinha, Edna
dc.contributor.authorMonteiro, Sandra M.
dc.contributor.authorVarandas, Simone
dc.contributor.authorSaavedra, Maria José
dc.date.accessioned2025-11-14T17:26:41Z
dc.date.available2025-11-14T17:26:41Z
dc.date.issued2025
dc.description.abstractCoastal environments have been recognized as key reservoirs for antibiotic-resistant bacteria. The present study evaluated marine gastropods, Phorcus lineatus, as potential bioindicators to assess the spread of antibiotic-resistant bacteria. P. lineatus was sampled in four sites, with different anthropogenic pressures, along the northwest Portuguese coastal area. From these specimens, bacteria were isolated and tested for their antimicrobial susceptibility, followed by their phylogenetic and pathotypic determination. All the Escherichia coli isolates showed resistance to at least one antimicrobial agent. The highest levels of multidrug resistance (25%) were observed in E. coli isolates obtained from SITE 2, which is impacted by the city of Porto and industrial settlements, while nearly 17% of these isolates showed a multiple antibiotic resistance (MAR) index higher than 0.2. Among the isolates, phylogroups A and B2 were the most prevalent, followed by phylogroup B1. The isolates of phylogroup A showed a higher prevalence of antimicrobial resistance. This study offers valuable insights into the antibiotic resistance risks posed to marine ecosystems and underscores the need for microbiological monitoring and the development of effective management strategies. The findings suggest P. lineatus as a potential bioindicator of antibiotic-resistant bacteria in marine environments.eng
dc.description.sponsorshipThis work is supported by National Funds by FCT (Portuguese Foundation for Science and Technology), under the projects UID/04033/2023 Centre for the Research and Technology of Agro-Environmental and Biological Sciences and LA/P/0126/2020 (https://doi.org/10.54499/LA/P/0126/2020); UIDB/00690/2020 (CIMO); 10.54499/UIDB/00211/2020 (CECA-ICETA) and LA/P/0007/2020 (SusTEC); ATLANTIDA project (ref. NORTE-01-0145-FEDER-000 040), also supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement and through the European Regional Development Fund (ERDF) and the Operational Competitiveness Programme (COMPETE); FCT-PhD grant (2023.04257.BDANA) attributed to Ana Rita Barata and FCT-PhD grant (2024.06170.BDANA) attributed to Ana Rita Pinto
dc.identifier.citationSantos, D.; Pinto, A.R.; Barata, R.; Fernandes, Conceição; Guedes, H.; Almeida, G.; Cabecinha, E.; Monteiro, S.M.; Varandas, S.; Saavedra, M.J. (2025). Emergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems-Gastropod Phorcus lineatus as a Bioindicator. Microbiology Research. ISSN 2036-7481. 16:6, p. 1-14
dc.identifier.doi10.3390/microbiolres16060133
dc.identifier.issn2036-7481
dc.identifier.urihttp://hdl.handle.net/10198/35081
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationInstitute for innovation, capacity building and sustainability of agri-food production
dc.relationMountain Research Center
dc.relationCenter for the Study of Animal Science
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relation.ispartofMicrobiology Research
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/
dc.subjectMarine gastropods
dc.subjectCoastal areas
dc.subjectAntibiotics susceptibility
dc.subjectPathotypes
dc.subjectEscherichia coli
dc.titleEmergence, Spread of Antimicrobial-Resistant Bacteria and Phylogenetic Relationships in Coastal Ecosystems—Gastropod Phorcus lineatus as a Bioindicatoreng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for innovation, capacity building and sustainability of agri-food production
oaire.awardTitleMountain Research Center
oaire.awardTitleCenter for the Study of Animal Science
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0126%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage14
oaire.citation.issue6
oaire.citation.startPage1
oaire.citation.titleMicrobiology Research
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFernandes
person.givenNameConceição
person.identifier15483
person.identifier.ciencia-id6514-3D41-4534
person.identifier.orcid0000-0003-2873-501X
person.identifier.scopus-author-id23018336600
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
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