Repository logo
 
Publication

Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa gomes

dc.contributor.authorSantos, Uilson Pereira dos
dc.contributor.authorCampos, Jaqueline Ferreira
dc.contributor.authorTorquato, Heron F. Vieira
dc.contributor.authorParedes-Gamero, Edgar Julian
dc.contributor.authorCarollo, Carlos Alexandre
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorSouza, Kely de Picoli
dc.contributor.authorSantos, Edson Lucas dos
dc.date.accessioned2018-01-31T10:00:00Z
dc.date.accessioned2018-01-31T15:40:35Z
dc.date.available2018-01-31T10:00:00Z
dc.date.available2018-01-31T15:40:35Z
dc.date.issued2016
dc.description.abstractThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-diphenyl-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2'-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis.en_EN
dc.description.sponsorshipThis work was supported by grants from FundacËão de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT, Brazil), Conselho Nacional de Desenvolvimento CientõÂfico e TecnoloÂgico (CNPq, Brazil) and PRODER, (24.073 ±A Ã , Portugal). E.L.S.; E.J.P.G e C.A.L.C., were recipient of felowship from CNPq, Brazil.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationSantos, Uilson P.; Campos, Jaqueline F.; Torquato, Heron Fernandes V.; Paredes-Gamero, Edgar Julian; Carollo, Carlos Alexandre; Estevinho, Leticia M.; Souza, Kely Picoli; Santos, Edson Lucas (2016). Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa gomes. PLoS ONE. ISSN 1932-6203. 11:12en_EN
dc.identifier.doi10.1371/journal.pone.0167531en_EN
dc.identifier.urihttp://hdl.handle.net/10198/15364
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.titleAntioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa gomesen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameEstevinho
person.givenNameLetícia M.
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.orcid0000-0002-9249-1948
person.identifier.scopus-author-id6506577664
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isAuthorOfPublication.latestForDiscovery4a1d5ba2-1854-4ca5-89a4-73f35e964df9

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
27.pdf
Size:
2.53 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Plain Text
Description: