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Authentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meats

dc.contributor.authorAmaral, Joana S.
dc.contributor.authorSantos, Cristina G.
dc.contributor.authorMelo, Vítor S.
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorMafra, Isabel
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-08T15:14:47Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-08T15:14:47Z
dc.date.issued2014
dc.description.abstractAlheira is a traditional meat product that is typical from the Northeast region of Portugal and much appreciated. It is a sort of sausage produced industrially or by small artisanal producers, having wheat bread and meats as main ingredients. Game meat Alheira (Alheira de caça) is considered one of the most attractive products since it should include different game meats. The aim of the present work was to identify the species of origin of meats added to game meat Alheira samples to verify their compliance with labelling. Species-specific PCR assays targeting mitochondrial genes of rabbit, hare, red deer, cow and pork were optimised and applied to industrial and artisanal samples. The assays revealed adequate specificity for each of the targeted species, with sensitivities of 0.01-0.1%. Results of the evaluation of 18 commercial samples identified several inconsistencies with labelling, namely the absence of declared game species (red deer, hare and rabbit) in ten samples and the presence of undeclared cow species in nine of the analysed samples. These findings indicate the occurrence of misleading labelling, suggesting the adulteration by substitution of game meats by cow meat to reduce production costs and the need to protect and valorise this kind of traditional food product.en_EN
dc.description.sponsorshipThe authors acknowledge the financial support of the University of Porto/Santander Totta “Projectos pluridisciplinares 2010” and the Fundação para a Ciência e a Tecnologia (FCT) through grant no. PEst-C/EQB/LA0006/2013.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationAmaral, Joana S.; Santos, Cristina G.; Melo, Vitor S.; Oliveira, M. Beatriz P.P.; Mafra, Isabel (2014). Authentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meats. Food Research International. ISSN 0963-9969. 60, p. 140-145en_EN
dc.identifier.doi10.1016/j.foodres.2013.11.003en_EN
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/16219
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAlheiraen_EN
dc.subjectAuthenticityen_EN
dc.subjectGame meaten_EN
dc.subjectMeat productsen_EN
dc.subjectPCRen_EN
dc.subjectSpecies identificationen_EN
dc.titleAuthentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meatsen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FEQB%2FLA0006%2F2013/PT
oaire.fundingStreamCOMPETE
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isProjectOfPublicationf7e58456-fee7-485f-8b49-d2c240945602
relation.isProjectOfPublication.latestForDiscoveryf7e58456-fee7-485f-8b49-d2c240945602

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