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Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

2014Journal article Open access
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dc.contributor.authorFaustino, Vera
dc.contributor.authorPinho, Diana
dc.contributor.authorYaginuma, Tomoko
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorLima, Rui A.
dc.date.accessioned2014-06-11T13:34:08Z
dc.date.available2014-06-11T13:34:08Z
dc.date.issued2014
dc.description.abstractRed blood cell (RBC) deformability has become one of the important factors to assess blood and cardiovascular diseases. The interest on blood studies have promoted a development of various microfluidic devices that treat and analyse blood cells. Recent years, besides the RBC deformability assessment, these devices are often applied to cancer cell detection and isolation from the whole blood. The devices for cancer cell isolation rely mainly on size and deformability of the cells. However, the examination of deformability of the RBCs mixed with cancer cells is lacking. This study aims at determining the deformation index (DI) of the RBCs in contact with cancer cells using a hyperbolic microchannel which generates a strong extensional flow. The DIs of human healthy RBCs and human RBCs in contact with a tumor cell line (HCT-15, colon carcinoma) were compared by analyzing the flowing RBCs images captured by a high speed camera. The results reveal that the RBCs that were in contact with HCT-15 cells have lower deformability than the normal RBCs.por
dc.description.sponsorshipThe authors acknowledge the financial support provided by: Student Mobility Placements with the program Lifelong Learning (Erasmus Program), 2007 Global COE Program “Global Nano-Biomedical Engineering Education and Research Network”, Japan. Grant-in-Aid for Science and Technology (PTDC/SAU-BEB/105650/2008, PTDC/EME-MFE/099109/2008 and PTDC/SAU-ENB/116929/2010) from the Science and Technology Foundation (FCT) and COMPETE, Portugal. The authors are also very grateful to Professor Mónica S.N. Oliveira (Strathclyde University), Professor Geyong M. Kim (University of Navarra) and Professor Sergio Arana (University of Navarra) for their discussion and suggestions to this research work.
dc.identifier.citationFaustino, V.; Pinho, D.; Yaginuma, T.; Calhelha, R. C.; Ferreira, I. C.F.R.; Lima, R. (2014). Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells. BioChip Journal. ISSN: 1976-0280. 8:1, p. 42-47por
dc.identifier.doi10.1007/s13206-014-8107-1
dc.identifier.urihttp://hdl.handle.net/10198/9678
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationANEURYSM - Hemodynamic Study in Cerebral Aneurysms
dc.relationBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
dc.subjectBlood on chipspor
dc.subjectRed blood cellspor
dc.subjectCell deformabilitypor
dc.subjectDeformation indexpor
dc.titleExtensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cellspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleANEURYSM - Hemodynamic Study in Cerebral Aneurysms
oaire.awardTitleBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F105650%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEME-MFE%2F099109%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PT
oaire.citation.endPage47por
oaire.citation.startPage42por
oaire.citation.titleBioChip Journalpor
oaire.citation.volume8por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
person.familyNamePinho
person.familyNameCalhelha
person.familyNameFerreira
person.familyNameLima
person.givenNameDiana
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.givenNameRui A.
person.identifier144781
person.identifier.ciencia-id5214-ED0C-35E8
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person.identifier.ciencia-idEE12-C3FB-349D
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0003-3428-637X
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.ridH-5157-2016
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id18437397800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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