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Orientador(es)
Resumo(s)
The quantification of total calcium, phosphorus, iron, chromium and nickel in cell culture medium by electrochemical or spectroscopic means may require digestion of samples. Nevertheless, when pH adjustment is performed for values higher than about 6.5, the formation of two phases occurs: a white precipitate and a clear solution. Analysing both phases using microelectrodes, atomic absorption spectrometry (AAS), diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy, X-ray dispersive (XRD) analysis, scanning electron microscopy (SEM) and energy dispersive spectroscopic (EDS) analysis, it was observed that iron, chromium and nickel are not co-precipitating with the white solid phase. If quantification of calcium, phosphorus and magnesium is intended, a ten-fold dilution at least, must be performed to avoid most of these elements going into the precipitate. This knowledge is crucial if a mineralization study is going to be made.
Descrição
Palavras-chave
Cell culture medium Microelectrodes Spectroscopic techniques
Contexto Educativo
Citação
Morais, S.; Carvalho, G.; Faria, Joaquim; Gomes, Helder; Sousa, João (1998). In vitro biomineralization by osteoblast-like cells : Part II - characterisation of cellular culture supernatants. Biomaterials. ISSN 0142-9612. 19:1-3, p. 23-29
Editora
Elsevier