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Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (coleoptera: Carabidae)

2018Artigo científico Acesso aberto
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dc.contributor.authorAlbertini, Alice
dc.contributor.authorSantos, Sónia A.P.
dc.contributor.authorMartins, Fátima
dc.contributor.authorPereira, J.A.
dc.contributor.authorLino-Neto, Teresa
dc.contributor.authorPetacchi, Ruggero
dc.contributor.authorBaptista, Paula
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2019-01-28T11:56:36Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2019-01-28T11:56:36Z
dc.date.issued2018
dc.description.abstractPest control service provided by natural enemies of Bactrocera oleae, the key pest of the olive tree, is nowadays recognized as fundamental. B. oleae has developed resistance to common insecticides, and negative effects both on consumers’ health and non-target species are the major drawbacks of conventional control strategies. Carabid beetles are potential B. oleae pupae predators, but their predation on field still need to be assessed. We tested adult Pseudoophonus rufipes, a species known to be active in olive orchard when pest pupae are abundant in the soil, in order to detect B. oleae pupae consumption at different post feeding times for both male and female carabids. An already existing protocol was used for detecting B. oleae mtDNA sequences of the cytochrome oxidase subunit I gene in carabids’ gut, and its versatility improved. B. oleae mtDNA was detected up to 20 h after pupa ingestion with a high percentage of success, without significant differences between sexes and pair primers used. Prey DNA extraction was tested from both dissected and non-dissected carabids, obtaining comparable results. The trapping system used to collect carabids for molecular assays and the new elements introduced in the protocol represent cost-effective solutions that may be beneficial for future laboratory trials and, mostly, for the analysis of field-collected predators. Fostering the investigation of soil predators in olive orchard may increase the design of conservation control strategies against B. oleae.
dc.description.sponsorshipScuola Superiore Sant’Anna, Pisa, Italy (PhD Programme in Agrobiodiversity).
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationAlbertini, Alice; Santos, Sónia A.P.; Martins, Fátima; Pereira, José A.; Lino-Neto, Teresa; Petacchi, Ruggero; Baptista, Paula (2018). Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (coleoptera: Carabidae). Spanish Journal of Agricultural Research. ISSN 1695-971X. 16, p. 1-9en_EN
dc.identifier.doi10.5424/sjar/2018163-12860en_EN
dc.identifier.issn1695-971X
dc.identifier.urihttp://hdl.handle.net/10198/18588
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectBiological controlen_EN
dc.subjectFruit flyen_EN
dc.subjectGut contenten_EN
dc.subjectOliveen_EN
dc.subjectPCRen_EN
dc.subjectPitfall trapen_EN
dc.subjectPost feeding timeen_EN
dc.titleDetection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (coleoptera: Carabidae)en_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNamePereira
person.familyNameBaptista
person.givenNameJosé Alberto
person.givenNamePaula
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.orcid0000-0002-2260-0600
person.identifier.orcid0000-0001-6331-3731
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57204366348
person.identifier.scopus-author-id14051688000
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublication.latestForDiscovery3f35226a-b17a-4f7d-8da1-3297105cbfe9

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