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Pomeganate leaf extracts characterization and their potential use as a functional ingredient

2023Conference object Open access
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dc.contributor.authorMarcelino, Sandra Andreia
dc.contributor.authorMandim, Filipa
dc.contributor.authorOludemi, Taofiq
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorFinimundy, Tiane C.
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-11-16T15:05:14Z
dc.date.available2023-11-16T15:05:14Z
dc.date.issued2023
dc.description.abstractThe pomegranate tree (Punica granatum L.) is indigenous to the Mediterranean region and is one of the oldest known plants. Studies have demonstrated their many biological and pharmaceutical benefits, which have been correlated to their bioactive content, namely secondary metabolites such as phenolic compounds [1]. However, 40 to 50% of the whole fruit is discarded, such as peel and leaves, originating valuable biowaste [2]. To support a circular economy and the prospective use of pomegranate biowaste as functional ingredients, the present research sought to ascertain the chemical composition and bioactive characteristics of pomegranate leaves. Maceration (ME), microwave (MAE), and ultrasound-assisted (UAE) extractions were the three extraction procedures chosen. HPLC-DAD-ESI/MS was used to identify and quantify the chemical composition of the various extracts. Two cell-based procedures were used to evaluate the antioxidant potential, TBARS and CAA. The antiproliferative potential was studied through sulforhodamine B colourimetric assays, using several tumour and a non-tumour cell lines. The extract's ability to reduce nitric oxide generation was used to determine its anti-inflammatory effects. Lastly, the microdilution technique was used to assess antimicrobial activity and cell viability assay conducted in two skin cell lines to support their possible use in topical formulation. According to the findings, the highest content of phenolic compounds was obtained in the MAE extract. Gallic acid, epicatechin, and granatin B were the most abundant compounds detected in all three extraction methodologies studied. All three extracts demonstrated lower IC50 values (0.83 - 1.70 g/mL) than the positive control Trolox (IC50 = 9.1 0.3 mg/mL), being the best results obtained for MAE and ME extracts. All extracts showed the capacity to halt the growth of tumour cell lines (GI50 between 19 and 76 g/mL), being gastric adenocarcinoma (AGS), the cell line with the highest sensitivity. All three extracts demonstrated broad-spectrum antibacterial inhibition, in which K. pneumoniae displayed the highest sensitivity to the extracts (MIC values of 0.6 mg/mL). In the tested skin cell lines, none of the studied extracts had any observable adverse effects, and more than 50% of viability was maintained after exposure to the highest tested concentrations of each extract (400 μg/mL). According to our findings, pomegranate leaves appear to be a source of health-promoting biomolecules. Those results sustain their exploitation and possible use by food and pharmaceutical industries in some biobased products, enhancing the circular economy and zero biowastes.pt_PT
dc.description.sponsorshipTo the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021); PhD grant of Filipa Mandim (SFRH/146614/2019), L. Barros and S. Heleno contracts. Also, to FEDER for financial support through the project OliveBIOextract (NORTE-01-0247-FEDER-049865). This work is also supported by MICINN supporting the Ramón y Cajal grant for M.A. Prieto (RYC-2017-22891) and JDC contract of T. Oludemi (FJC2019-042549-I).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarcelino, Sandra; Mandim, Filipa; Oludemi, Taofiq; Heleno, Sandrina A.; Finimundy, Tiane C.; Prieto, Miguel A.; Barros, Lillian (2023). Pomeganate leaf extracts characterization and their potential use as a functional ingrediente. In 8th Portuguese Young Chemistry Meeting. Vila Realpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/28890
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPomegranate treept_PT
dc.titlePomeganate leaf extracts characterization and their potential use as a functional ingredientpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.conferencePlaceVila Realpt_PT
oaire.citation.title8th Portuguese Young Chemistry Meetingpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMarcelino
person.familyNameMandim
person.familyNameOludemi
person.familyNameHeleno
person.familyNameFinimundy
person.familyNamePrieto Lage
person.familyNameBarros
person.givenNameSandra Andreia
person.givenNameFilipa
person.givenNameTaofiq
person.givenNameSandrina A.
person.givenNameTiane C.
person.givenNameMiguel A.
person.givenNameLillian
person.identifier107688
person.identifier469085
person.identifier.ciencia-idF318-9049-EB99
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person.identifier.ciencia-id221E-1E00-AB74
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0003-1490-9445
person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0003-2822-3532
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0003-3516-0795
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridL-5951-2014
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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