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A passive microfluidic device based on crossflow filtration for cell separation measurements: a spectrophotometric characterization

2018Journal article Open access
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dc.contributor.authorFaustino, Vera
dc.contributor.authorCatarino, Susana
dc.contributor.authorPinho, Diana
dc.contributor.authorLima, Rui A.
dc.contributor.authorMinas, Graça
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2019-02-04T14:36:57Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2019-02-04T14:36:57Z
dc.date.issued2018
dc.description.abstractMicrofluidic devices have been widely used as a valuable research tool for diagnostic applications. Particularly, they have been related to the successful detection of different diseases and conditions by assessing the mechanical properties of red blood cells (RBCs). Detecting deformability changes in the cells and being able to separate those cells may be a key factor in assuring the success of detection of some blood diseases with diagnostic devices. To detect and separate the chemically modified RBCs (mimicking disease-infected RBCs) from healthy RBCs, the present work proposes a microfluidic device comprising a sequence of pillars with different gaps and nine different outlets used to evaluate the efficiency of the device by measuring the optical absorption of the collected samples. This latter measurement technique was tested to distinguish between healthy RBCs and RBCs chemically modified with glutaraldehyde. The present study indicates that it was possible to detect a slight differences between the samples using an optical absorption spectrophotometric setup. Hence, the proposed microfluidic device has the potential to perform in one single step a partial passive separation of RBCs based on their deformability.en_EN
dc.description.sponsorshipResearch supported by FCT with the reference projects POCI-01-0145-FEDER-016861 (PTDC/QEQ-FTT/4287/2014), NORTE-01-0145-FEDER-029394 (PTDC/EMD-EMD/29394/2017), NORTE-01-0145-FEDER-030171 (PTDC/EME-SIS/30171/2017), UID/EEA/04436/2013, by FEDER funds through the COMPETE 2020, NORTE2020, PORTUGAL2020—Programa Operacional Competitividade e Internacionalização (POCI) with the reference project POCI-01-0145-FEDER-006941 and by the NORTE-01-0145-FEDER-028178 (PTDC/EEI-EEE/28178/2017) project, funded 85% from Programa Operacional Regional do Norte and 15% from FCT. V.F., S.O.C. and D.P. thank, respectively, the FCT for the grants SFRH/BD/99696/2014, SFRH/BPD/108889/2015 and SFRH/BD/89077/2012 supported by national funds from Ministérios da Ciência, Tecnologia e Ensino Superior and by FSE through the POCH—Programa Operacional Capital Humano.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFaustino, Vera; Catarino, Susana O.; Pinho, Diana; Lima, R.; Minas, Graça (2018). A passive microfluidic device based on crossflow filtration for cell separation measurements: A spectrophotometric characterization. Biosensors. ISSN 2079-6374. 8:4en_EN
dc.identifier.doi10.3390/bios8040125en_EN
dc.identifier.issn2079-6374
dc.identifier.urihttp://hdl.handle.net/10198/18632
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationNORTE-01-0145-FEDER-030171
dc.relationPOCI-01-0145-FEDER-006941
dc.relationMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
dc.relationMultifunctional lab-on-a-chip for blood properties detection with integrated PVDF acoustic transducers: application to malaria biomarkers
dc.subjectCross-flow filtrationen_EN
dc.subjectMicrofluidicsen_EN
dc.subjectRed blood cellsen_EN
dc.subjectSeparationen_EN
dc.subjectSpectrophotometryen_EN
dc.titleA passive microfluidic device based on crossflow filtration for cell separation measurements: a spectrophotometric characterizationen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
oaire.awardTitleMultifunctional lab-on-a-chip for blood properties detection with integrated PVDF acoustic transducers: application to malaria biomarkers
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FEME-SIS%2F30171%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F99696%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F108889%2F2015/PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamFARH
oaire.fundingStreamSFRH
person.familyNamePinho
person.givenNameDiana
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.orcid0000-0002-3884-6496
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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