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The aim of this study was to determine the palynological origin, phenolic
content, antioxidant properties and antibacterial activity of honeybee-
collected pollen from five Portuguese Natural-Parks [Parque Nacional
Peneda Geres (PNPG); Parque Natural do Montesinho (PNM); Parque
Natural do Alvao (PNA); Parque Natural da Serra da Estrela (PNSE) and
Parque Natural do Douro Internacional (PNDI)].
Eight families were found in the mixture of bee pollen: Rosaceae, Cistaceae,
Boraginaceae, Asteraceae, Fagaceae, Ericaeae, Myrtaceae and Fabaceae. The
phenolic compounds content, determined by the Folin-Ciocalteu method,
varied between 10.5 and 16.8 mg of gallic acid equivalents/ g of extract (mg
GAE/ g) found in bee pollen from PNM and PNDI, respectively. The free
radical scavenging measured showed the highest effective extract - PNM
with ECSO 2.16, followed by PND with 2.24 mg/ mL. In the 0-carotene
bleaching assay the same behaviour as in the DPPH method was verified.
We also verified that the presence of pollen differentially affected the
growth of bacteria Gram-positive, Gram-negative and fetmentative yeasts
under study, depending on the microorganism and the type of bee pollen
used. This is an important study since, as far we know, it is the first time that
Portuguese bee pollen from Natural Parks was studied, and their characterization
can increase their economic value.
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Dias, Teresa; Morais, Margarida; Moreira, Leandro; Estevinho, Leticia M. (2011). Honeybee-collected pollen from five Portuguese Natural Parks: palynological origin, phenolic content, antioxidant properties and antimicrobial activity. In I congresso Ibérico de Apicultura. Castelo Branco. ISBN 978-989-8196-15-6