Repository logo
 
Publication

Vespa velutina: using microsatellites to compare the genetic diversity of founding nests in the leading-edge populations in Portugal

2023Conference object Open access
Simple item page
dc.contributor.authorCosta, Maíra
dc.contributor.authorQuaresma, Andreia
dc.contributor.authorDoblas-Bajo, Mónica
dc.contributor.authorHenriques, Dora
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2024-02-08T12:17:08Z
dc.date.available2024-02-08T12:17:08Z
dc.date.issued2023
dc.description.abstractThe invasive yellow-legged hornet (Vespa velutina nigrithorax) is a voracious predator of honeybees and other pollinators, its diffusion generates relevant environmental and socio-economic impacts. Native from China, it rapidly started spreading throughout Europe, after its first sight in France in 2004. V. v. nigrithorax was first reported in Portugal, in the region of Viana do Castelo, in 2011 and since then it has been spreading through the country, with Bragança representing the Northeastern edge of its distribution in Portugal. The aim of this study was to compare the genetic diversity between nests collected in Viana do Castelo and Bragança. Four nests were analysed, two from Viana do Castelo and two from Bragança. Each sample nest is represented by 30 workers making up a total of 120 individuals analysed for this study. Total DNA was extracted from the thorax of each individual using the Nucleospin® Tissue (Macherey-Nagel). The individuals were genotyped using 16 microsatellite loci divided into three multiplex combinations. The lengths of the fragments were determined using GeneMapper 3.7 (Applied Biosystems). Genetic diversity statistics, which included observed number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and unbiased expected heterozygosity (uHe) were estimated using GENALEX 6.5. This software was also implemented to assess genetic structure using a Principal Coordinate Analyses (PCoA). Our results show that both nests from both origins have low genetic diversity, with Bragança showing higher genetic diversity (Na=2; Ne= 1.600; Ho=0.427; He=0.330; uHe=0.333) than Viana do Castelo (Na=1.750; Ne=1.482; Ho=0.363; He=0.258; uHe=0.260). The genetic structure show that distinct groups might be in the origin of both nests’ location. These results suggest that the population of Vespa velutina from the region of Bragança may be an expansion from multiple source populations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, Maíra; Quaresma, Andreia; Doblas-Bajo, Mónica; Henriques, Dora; Pinto, M. Alice (2023). Vespa velutina: using microsatellites to compare the genetic diversity of founding nests in the leading-edge populations in Portugal. In TIBE - Invaders on the Horizon. Vila do Condept_PT
dc.identifier.urihttp://hdl.handle.net/10198/29454
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVeslpa velutinapt_PT
dc.subjectGenetic diversitypt_PT
dc.subjectMicrosatellitept_PT
dc.titleVespa velutina: using microsatellites to compare the genetic diversity of founding nests in the leading-edge populations in Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceVila do Condept_PT
oaire.citation.titleTIBE - Invaders on the Horizonpt_PT
person.familyNameCosta
person.familyNameQuaresma
person.familyNameHenriques
person.familyNamePinto
person.givenNameMaíra
person.givenNameAndreia
person.givenNameDora
person.givenNameM. Alice
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-id4F1A-4E4A-3F23
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0002-8678-5800
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id57119742600
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id8085507800
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationc7b97b29-3139-43ff-ae85-23f613e280ed
relation.isAuthorOfPublicationd417b0ac-c8ee-473a-a355-820b5b9a3f55
relation.isAuthorOfPublicationd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication.latestForDiscoveryd2abd09f-a90c-4cfb-9a60-7fc32f56184d

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
TIBE2023.pdf
Size:
308.64 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus