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Headspace solid-phase microextraction and gas chromatography/ion trap-mass spectrometry applied to a living system: Pieris brassicae fed with kale

dc.contributor.authorFernandes, Fátima
dc.contributor.authorPereira, David M.
dc.contributor.authorPinho, P. Guedes de
dc.contributor.authorValentão, Patrícia
dc.contributor.authorPereira, J.A.
dc.contributor.authorBento, Albino
dc.contributor.authorAndrade, Paula B.
dc.date.accessioned2011-01-14T10:09:44Z
dc.date.available2011-01-14T10:09:44Z
dc.date.issued2009
dc.description.abstractThe influence of Pieris brassicae feeding on kale was monitored, by evaluating its effect on the volatiles released by the plant through time. This is the first study applying headspace solid-phase microextraction (HS-SPME) and gas chromatography/ion trap-mass spectrometry to an isolated insect, as most studies analyse the insect–plant system as a whole, being unable to evaluate the contribution of the insect itself. Substantial differences were noticed between the volatiles composition of kale before and after the insect’s attack. More than 60 compounds were found, including terpenes, lipoxygenase pathway by-products, ketones, norisoprenoids, etc. After insect attack, l-camphor, sabinene and a-thujene were found and limonene and eucalyptol suffered a noticeable increase. A considerable rise in (Z)-3-hexenyl acetate was also observed. In vivo accumulation of limonene and camphor by the insect was detected. The findings contribute to the knowledge of the ecological interactions between the two species.por
dc.description.sponsorshipFCTpor
dc.identifier.citationFernandes, F.; Pereira, D.; Guedes de Pinho, P.; Valentão, P.; Pereira, J.A.; Bento, A.; Andrade, P.B. (2010). Headspace solid-phase microextraction and gas chromatography/ion trap-mass spectrometry applied to a living system: Pieris brassicae fed with kale. Food Chemistry. ISSN 0308-8146. 119:4, p. 1681-1693por
dc.identifier.doi10.1016/j.foodchem.2009.09.046
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/3112
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationPieris brassicae as laboratory of synthesis of new compounds with biological potential, starting from Brassica oleracea var. costata, Brassica oleracea var. acephala and Brassica rapa var. rapa.
dc.subjectBrassica oleracea L. var. acephalapor
dc.subjectKalepor
dc.subjectPieris brassicaepor
dc.subjectVolatile compoundspor
dc.subjectHS-SPMEpor
dc.subjectGC/IT-MSpor
dc.titleHeadspace solid-phase microextraction and gas chromatography/ion trap-mass spectrometry applied to a living system: Pieris brassicae fed with kalepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePieris brassicae as laboratory of synthesis of new compounds with biological potential, starting from Brassica oleracea var. costata, Brassica oleracea var. acephala and Brassica rapa var. rapa.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-AAM%2F64150%2F2006/PT
oaire.citation.endPage1693por
oaire.citation.issue119(4)por
oaire.citation.startPage1681por
oaire.citation.titleFood Chemistrypor
oaire.fundingStream5876-PPCDTI
person.familyNamePereira
person.familyNameBento
person.givenNameJose Alberto
person.givenNameAlbino António
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.ciencia-idD516-325A-9AD7
person.identifier.orcid0000-0002-2260-0600
person.identifier.orcid0000-0001-5215-785X
person.identifier.ridL-6798-2014
person.identifier.ridN-9706-2016
person.identifier.scopus-author-id57204366348
person.identifier.scopus-author-id35247694000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication233115be-9d46-49d0-8b7d-2d64406d64a0
relation.isAuthorOfPublication.latestForDiscovery7932162e-a2da-4913-b00d-17babbe51857
relation.isProjectOfPublicationf81a81fb-2f99-45fe-bab7-2267d47bf7f3
relation.isProjectOfPublication.latestForDiscoveryf81a81fb-2f99-45fe-bab7-2267d47bf7f3

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