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Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

2018Journal article Open access
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dc.contributor.authorFinimundy, Tiane C.
dc.contributor.authorAbreu, Rui M.V.
dc.contributor.authorBonetto, Natalia
dc.contributor.authorScariot, Fernando J.
dc.contributor.authorDillon, Aldo J.P.
dc.contributor.authorEcheverrigaray, Sergio
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorHenriques, João A.P.
dc.contributor.authorRoesch-Ely, Mariana
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2019-01-28T11:48:24Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2019-01-28T11:48:24Z
dc.date.issued2018
dc.description.abstractPleurotus sajor-caju (PSC) is an edible mushroom used in food supplements, presenting antitumor properties through induction of cell death pathways. The PSC potential against colorectal cancer was analyzed by exposing HCT116wt cells to different PSC extracts. The PSC n-hexane extract (PSC-hex) showed the highest cytotoxicity effect (IC50 value 0.05 mg/mL). The observed cytotoxicity was then associated to apoptosis-promoting and cell cycle-arrest pathways. PSC-hex was able to induce apoptosis related to breakdown of mitochondrial membrane potential and ROS generation. The absence of cytotoxicity in HTC116-p53 and HTC116-Bax cells, alongside with an increase in p53, Bax and Caspase-3 expression, and decrease in Bcl-2 expression, supports that the proapoptotic effect is probably induced through a p53 associated pathway. PSC-hex induced cell cycle arrest at G2/ M in HCT116wt without cytotoxicity in HTC116-p21 cells. These findings suggest that a p21/p53 cell cycle regulation pathway is probably disrupted by compounds present on PSC-hex. Identification of the major components was then performed with ergosta-5,7,22-trien-3β-ol representing 30.6% of total weight. In silico docking studies of ergosta-5,7,22-trien-3β against Bcl-2 were performed and results show a credible interaction with the Bcl-2 hydrophobic cleft. The results show that PSC-hex can be used as supplementary food for adjuvant therapy in colorectal carcinoma.
dc.description.sponsorshipThe authors thank Dr. Larsen (Institut National de la Santé et de la Recherche Médicale) for the gift of HCT116-Bax, HCT116-p21 and HCT116-p53 cells. The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros contract. T.C. Finimundy thanks CAPES Foundation, Ministry of Education of Brazil (CAPES fellow, process number 88881.134581/ 2016–01). This work is supported by a grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFinimundy, Tiane C.; Abreu, Rui M.V.; Bonetto, Natalia; Scariot, Fernando J.; Dillon, Aldo J.P.; Echeverrigaray, Sergio; Barros, Lillian; Ferreira, Isabel C.F.R.; Henriques, João A.P.; Roesch-Ely, Mariana (2018). Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines. Food and Chemical Toxicology. ISSN 0278-6915. 112, p. 383-392en_EN
dc.identifier.doi10.1016/j.fct.2018.01.015en_EN
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10198/18555
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectApoptosisen_EN
dc.subjectCytotoxicityen_EN
dc.subjectPleurotus sajor-cajuen_EN
dc.subjectSterolsen_EN
dc.titleApoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell linesen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameFinimundy
person.familyNameAbreu
person.familyNameBarros
person.familyNameFerreira
person.givenNameTiane C.
person.givenNameRui M.V.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id2F18-67B1-776A
person.identifier.ciencia-id0F19-0DE2-12A2
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0003-3516-0795
person.identifier.orcid0000-0002-7745-8015
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id7003290613
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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