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Unravelling the effect of control agents on Gnomoniopsis smithogilvyi on a chestnutā€based medium by proteomics

2023Journal article Open access
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dc.contributor.authorƁlvarez Rubio, Micaela
dc.contributor.authorAgostini, Isadora
dc.contributor.authorSampaio, Ana
dc.contributor.authorRomƔn, Ɓngel-Carlos
dc.contributor.authorDelgado, JosuƩ
dc.contributor.authorRodrigues, Paula
dc.date.accessioned2024-03-21T11:47:53Z
dc.date.available2024-03-21T11:47:53Z
dc.date.issued2023
dc.description.abstractGnomoniopsis smithogilvyi is the major chestnut pathogen, responsible for economic losses and recently described as a 3-nitropropionic acid and diplodiatoxin mycotoxin producer. Bacillus amyloliquefaciens QST 713 (SerenadeĀ® ASO), B. amyloliquefaciens CIMO-BCA1, and the fungicide HorizonĀ® (tebuconazole) have been shown to reduce the growth of G. smithogilvyi. However, they enhanced mycotoxin production. Proteomics can clarify the mould's physiology and the impact of antifungal agents on the mould's metabolism. Thus, the aim of this study was to assess the impact of HorizonĀ®, SerenadeĀ®, and B. amyloliquefaciens CIMO-BCA1 in the proteome of G. smithogilvyi to unveil their modes of action and decipher why the mould responds by increasing the mycotoxin production. For this, themyceliumclose to the inhibition zone provoked by antifungals was macroscopically and microscopically observed. Proteins were extracted and analysed using a Q-Exactive plus Orbitrap. RESULTS: The results did not elucidate specific proteins involved in the mycotoxin biosynthesis, but these agents provoked different kinds of stress on the mould, mainly affecting the cell wall structures and antioxidant response, which points to the mycotoxins overproduction as a defence mechanism. The biocontrol agent CIMO-BCA1 acts similar to tebuconazole. The results revealed different responses on the mould's metabolism when co-cultured with the two B. amyloliquefaciens, showing different modes of action of each bacterium, which opens the possibility of combining both biocontrol strategies. CONCLUSION: These results unveil different modes of action of the treatments that could help to reduce the use of toxic chemicals to combat plant pathogens worldwide.pt_PT
dc.description.sponsorshipThis work was funded by the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 through financial support to projects GnomoCastRot (CMFPE3; EXPL2021CIMO_02), CIMO (UID/AGR/00690/2020), SusTEC (LA/P/0007/2020), CITAB (UID/AGR/04033/2020), and Inov4Agro (LA/P/0126/2020). M. Ɓlvarez was the recipient of grant BES- 2017-081340 funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future, and Grant Margarita Salas MS-14 was funded by the Ministerio de Universidades and European Union NextGenerationEU. The Q-Exactive mass spectrometer to proteomic research was acquired by the grant UNEX-AE-3394 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationƁlvarez, Micaela; Agostini, Isadora; Sampaio, Ana; RomĆ”n, Ɓngel-Carlos; Delgado, JosuĆ©; Rodrigues, Paula (2023). Unravelling the effect of control agents on Gnomoniopsis smithogilvyi on a chestnutā€based medium by proteomics. Pest Management Science. ISSN 1526-498X. 80:4, p. 1895ā€“1903pt_PT
dc.identifier.doi10.1002/ps.7920pt_PT
dc.identifier.eissn1526-4998
dc.identifier.issn1526-498X
dc.identifier.urihttp://hdl.handle.net/10198/29640
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sonspt_PT
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationInstitute for innovation, capacity building and sustainability of agri-food production
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBrown rotpt_PT
dc.subjectBacillus amyloliquefacienspt_PT
dc.subjectTebuconazolept_PT
dc.subjectProteomicspt_PT
dc.titleUnravelling the effect of control agents on Gnomoniopsis smithogilvyi on a chestnutā€based medium by proteomicspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardTitleInstitute for innovation, capacity building and sustainability of agri-food production
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0126%2F2020/PT
oaire.citation.titlePest Management Sciencept_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRodrigues
person.givenNamePaula
person.identifier.ciencia-id2312-18AE-DA42
person.identifier.orcid0000-0002-3789-2730
person.identifier.scopus-author-id7102639646
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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