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Phenolic compounds and bioactivity of Cytisus villosus Pourr.

2018Artigo científico Acesso aberto
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dc.contributor.authorBouziane, Amel
dc.contributor.authorBakchiche, Boulanouar
dc.contributor.authorDias, Maria Inês
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorAlSalamat, Husam A.
dc.contributor.authorBardaweel, Sanaa K.
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2019-02-04T17:11:10Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2019-02-04T17:11:10Z
dc.date.issued2018
dc.description.abstractThe present study focuses on the chemical composition, antioxidant, antimicrobial, and anti proliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Cytisus villosus Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatechin dimer the major compound (111 ± 5 µg/g·dw) in the aqueous extract, while myricetin-O-rhamnoside(226±9 µg/g·dw) was the main molecule in the ethyl acetate extract. Both extracts exhibited good scavenging activities against DPPH radical(EC50 µg/mLof59±2and31±2 for aqueous and ethyl acetate extracts,respectively). However,the ethyl acetate extract demonstrated more potent quenching activities than the aqueous extract.The antimicrobial activities were assessed on selected Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, as well as on pathogenic fungus Candida glabrata. The extracts possessed selective and potent antimicrobial activities against the Gram-positive bacterium (IC50 of 186±9 µg/mL and 92±3 µg/mL for aqueous and ethyl acetate extracts, respectively).Finally, C. villosus extracts were evaluated for their antiproliferative potential on three human cancer cell lines representing breast and colon cancers. Although both extracts demonstrated sufficient growth inhibition of the threed ifferent cell lines,the ethyl acetate extract exhibited higher activity(LD50 values of1.57±0.06mg/mL,2.2±0.1mg/mL,and3.2±0.2mg/mLforT47D,MCF-7,andHCT-116cell lines). Both the extracts obtained from the aerial parts of C.villosus revealed very promising results and could be applied as functional agents in the food,pharmaceutical,and cosmeceutical industries.en_EN
dc.description.sponsorshipThe authors are grateful to the University of Jordan-Deanship of Academic Research (Jordan), the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros contract. The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E. APC was sponsored by MDPI.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationBouziane, Amel; Bakchiche, Boulanouar; Dias, Maria Inês; Barros, Lillian; Ferreira, Isabel C.F.R.; AlSalamat, Husam A.; Bardaweel, Sanaa K. (2018). Phenolic compounds and bioactivity of Cytisus villosus Pourr.. Molecules. ISSN 1420-3049. 23, p. 1-12en_EN
dc.identifier.doi10.3390/molecules23081994en_EN
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10198/18737
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAntimicrobialen_EN
dc.subjectAntioxidanten_EN
dc.subjectAntiproliferative activityen_EN
dc.subjectCytisus villosusen_EN
dc.subjectPhytochemicalen_EN
dc.titlePhenolic compounds and bioactivity of Cytisus villosus Pourr.en_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/AGR/00690/2013
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameDias
person.familyNameBarros
person.familyNameFerreira
person.givenNameMaria Inês
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication7ca8432b-e7f4-4116-a56e-9eba0d4403bd
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscovery7ca8432b-e7f4-4116-a56e-9eba0d4403bd
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