Haemocompatibility of iron oxide nanoparticles synthesized for theranostic applications: a high-sensitivity microfluidic tool

Rodrigues, Raquel Oliveira; Bañobre-López, Manuel; Gallo, Juan; Tavares, Pedro B.; Silva, Adrián; Lima, Rui A.; Gomes, Helder

Publication

Haemocompatibility of iron oxide nanoparticles synthesized for theranostic applications: a high-sensitivity microfluidic tool

2016Journal article

dc.contributor.author	Rodrigues, Raquel Oliveira
dc.contributor.author	Bañobre-López, Manuel
dc.contributor.author	Gallo, Juan
dc.contributor.author	Tavares, Pedro B.
dc.contributor.author	Silva, Adrián
dc.contributor.author	Lima, Rui A.
dc.contributor.author	Gomes, Helder
dc.date.accessioned	2016-08-30T09:22:21Z
dc.date.available	2016-08-30T09:22:21Z
dc.date.issued	2016
dc.description.abstract	The poor heating efficiency of the most reported magnetic nanoparticles (MNPs), allied to the lack of comprehensive biocompatibility and haemodynamic studies, hampers the spread of multifunctional nanoparticles as the next generation of therapeutic bio-agents in medicine. The present work reports the synthesis and characterization, with special focus on biological/toxicological compatibility, of superparamagnetic nanoparticles with diameter around 18 nm, suitable for theranostic applications (i.e. simultaneous diagnosis and therapy of cancer). Envisioning more insights into the complex nanoparticle-red blood cells (RBCs) membrane interaction, the deformability of the human RBCs in contact with magnetic nanoparticles (MNPs) was assessed for the first time with a microfluidic extensional approach, and used as an indicator of haematological disorders in comparison with a conventional haematological test, i.e. the haemolysis analysis. Microfluidic results highlight the potential of this microfluidic tool over traditional haemolysis analysis, by detecting small increments in the rigidity of the blood cells, when traditional haemotoxicology analysis showed no significant alteration (haemolysis rates lower than 2 %). The detected rigidity has been predicted to be due to the wrapping of small MNPs by the bilayer membrane of the RBCs, which is directly related to MNPs size, shape and composition. The proposed microfluidic tool adds a new dimension into the field of nanomedicine, allowing to be applied as a highsensitivity technique capable of bringing a better understanding of the biological impact of nanoparticles developed for clinical applications.	pt_PT
dc.identifier.citation	Rodrigues, Raquel O.; Bañobre-López, Manuel; Gallo, Juan; Tavares, Pedro B.; Silva, Adrián M. T.; Lima, R.; Gomes, Helder (2016). Haemocompatibility of iron oxide nanoparticles synthesized for theranostic applications: a high-sensitivity microfluidic tool. Journal of Nanoparticle Research. ISSN 1388-076418. 18:7, p.1-17	pt_PT
dc.identifier.doi	10.1007/s11051-016-3498-7	pt_PT
dc.identifier.issn	1388-0764
dc.identifier.uri	http://hdl.handle.net/10198/13169
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Iron oxide nanoparticles	pt_PT
dc.subject	Haemocompatibility	pt_PT
dc.subject	RBCs deformation	pt_PT
dc.subject	Microfluidic device	pt_PT
dc.subject	Magnetic hyperthermia	pt_PT
dc.subject	MRI	pt_PT
dc.subject	Superparamagnetism	pt_PT
dc.title	Haemocompatibility of iron oxide nanoparticles synthesized for theranostic applications: a high-sensitivity microfluidic tool	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	17	pt_PT
oaire.citation.issue	7	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	Journal of Nanoparticle Research	pt_PT
oaire.citation.volume	18	pt_PT
person.familyName	Lima
person.familyName	Gomes
person.givenName	Rui A.
person.givenName	Helder
person.identifier.ciencia-id	EE12-C3FB-349D
person.identifier.ciencia-id	6218-1E19-13EE
person.identifier.orcid	0000-0003-3428-637X
person.identifier.orcid	0000-0001-6898-2408
person.identifier.rid	H-5157-2016
person.identifier.scopus-author-id	18437397800
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	article	pt_PT
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