Publication
Quantitative risk assessment of listeriosis from traditional brazilian minas artisanal semi-hard and fresh soft cheeses
| dc.contributor.author | Campagnollo, Fernanda B. | |
| dc.contributor.author | Cadavez, Vasco | |
| dc.contributor.author | Gonzales-Barron, Ursula | |
| dc.contributor.author | Sant’Ana, Anderson | |
| dc.contributor.author | Schaffner, Donald W. | |
| dc.date.accessioned | 2019-02-28T10:31:58Z | |
| dc.date.available | 2019-02-28T10:31:58Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Traditional Minas cheeses are very popular in Brazil. These cheeses are of two types: artisanal ripened semi-hard cheeses (HC) produced with raw milk and refrigerated fresh soft cheeses (SC) generally produced with pasteurized milk. Purpose: This study estimates the risk of listeriosis due to HC and SC consumption using quantitative microbial risk assessment (QMRA). Methods: QMRAs were developed for both cheese types. The HC model contained a module for predicting Listeria monocytogenes decline during ripening. The SC model contained a refrigerated storage module for predicting L. monocytogenes growth during storage. HC modeling scenarios varied L. monocytogenes starting concentration over -2.4 to 6 log CFU/ml in raw milk and three ripening times (4, 22, and 60 days). SC modeling scenarios varied L. monocytogenes starting concentration (-2.4 to 4 log CFU/ml in milk). Inclusion of antilisterial lactic acid bacteria (LAB) was also examined. Simulations (100,000 iterations per scenario) were carried out using the @Risk add-in for Excel. Results: Aging HC reduced risk, and risk was influenced by L. monocytogenes starting concentration. Aging 22 days with inhibitory LAB reduced risk more than 4 million-fold when L. monocytogenes was assumed to be 6 log CFU/ml in raw milk, and was lower risk than HC made without LAB and with a starting concentration of 1 log CFU/ml in raw milk. Inclusion of inhibitory LAB reduced risk of listeriosis from SC, but not as dramatically as for HC. Relative risk to vulnerable populations reduced 4.4 and 3.4-fold when L. monocytogenes starting concentrations were 1 log and 4 log CFU/ml in milk, respectively. Significance: The results of the QMRA predict that consumption of contaminated Minas cheeses can present a high risk of listeriosis, especially for vulnerable populations. Scenario analyses indicate that aging of HC and inclusion of LAB in HC and SC are effective risk mitigation measures. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Campagnollo, Fernanda; Cadavez, Vasco; Gonzales-Barron, Ursula A.; Sant’Ana, Anderson; Schaffner, Donald (2018). Quantitative risk assessment of listeriosis from traditional brazilian minas artisanal semi-hard and fresh soft cheeses. In IAFP 2018 Annual Meeting. Salt Lake City, Utah | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/19033 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Risk assessment of Listeriosis | pt_PT |
| dc.subject | Semi-hard cheeses | pt_PT |
| dc.subject | Fresh soft cheeses | pt_PT |
| dc.title | Quantitative risk assessment of listeriosis from traditional brazilian minas artisanal semi-hard and fresh soft cheeses | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Salt Lake City, Utah | pt_PT |
| oaire.citation.title | IAFP 2018 Annual Meeting | pt_PT |
| person.familyName | Cadavez | |
| person.familyName | Gonzales-Barron | |
| person.givenName | Vasco | |
| person.givenName | Ursula | |
| person.identifier | R-000-HDG | |
| person.identifier.ciencia-id | 441B-01AB-A12E | |
| person.identifier.ciencia-id | 0813-C319-B62A | |
| person.identifier.orcid | 0000-0002-3077-7414 | |
| person.identifier.orcid | 0000-0002-8462-9775 | |
| person.identifier.rid | A-3958-2010 | |
| person.identifier.scopus-author-id | 9039121900 | |
| person.identifier.scopus-author-id | 9435483700 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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