Repository logo
 
Publication

Its2 metabarcoding: a promising approach for identification of botanical origin of bee-collected pollen

2021Conference object Open access
Simple item page
dc.contributor.authorQuaresma, Andreia
dc.contributor.authorVan der Steen, Joseph
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorBiron, David G.
dc.contributor.authorBrodschneider, Robert
dc.contributor.authorBrusbardis, Valters
dc.contributor.authorCarreck, Norman L.
dc.contributor.authorMary, Frances Coffey
dc.contributor.authorFormato, Giovanni
dc.contributor.authorGraaf, Dirk C.
dc.contributor.authorGratzer, Kristina
dc.contributor.authorHatjina, Fani
dc.contributor.authorKilpinem, Ole
dc.contributor.authorKeller, Alexander
dc.contributor.authorLaget, Dries
dc.contributor.authorPietropaoli, Marco
dc.contributor.authorRufino, José
dc.contributor.authorVejsnaes, Flemming
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2023-05-22T13:05:17Z
dc.date.available2023-05-22T13:05:17Z
dc.date.issued2021
dc.description.abstractBee products have long been used in human’s diet and their consumption has increasingly been recognized has beneficial for human’s health. One such product is pollen, which is a particularly interesting food as it contains bioactive compounds and all the essential amino-acids needed by humans. However, the composition of bee-collected pollen depends on the environment where the visited plants grow (e.g.: climatic conditions, soil type) and, above all, on the plant species [1]. Therefore, identification of the botanical origin of bee-collected pollen is important for a fuller characterization of this food product. Until recently, pollen identification has been carried out using light microscopy, a costly approach that often provides low taxonomic resolution. However, with high-throughput sequencing (HTS) becoming increasingly affordable, DNA metabarcoding is emerging as a promising alternative to light microscopy. In addition to be time- and cost-effective for large sample sizes, metabarcoding has the potential to allow identification of pollen mixtures at the species level. However, before it can be widely employed in pollen analysis, the reliability of this molecular tool must be appraised. Herein, we compared the relative abundances obtained by the two approaches on 108 bee-collected pollen samples from 10 European countries. To that end, the 108 samples were first homogenized and split into two identical sub-sets. One sub-set was analysed by palynology experts from the “Institut für Bienenkunde”, Germany, and the other one was subjected to HTS, using ITS2 as the barcode, in the labs of CIMO and CIBIO. Pairwise comparisons of the relative abundances at the family level of the 108 samples show no significant differences (P ≥ 0.1057, Wilcoxon signed-rank test) and high correlation values (0.2736 ≤ r ≤ 0.9842, Pearson’s correlation) between the two approaches. The highest correlation values were observed for Italian samples (0.7245 ≤ r ≤ 0.9842; global r = 0.8958) and the lowest for Greek samples (0.0266 ≤ r ≤ 0.9703; global r = 0.4929). Despite, the few outliers, which can be improved by further optimization of the protocols, these results suggest that ITS2 metabarcoding promises to be a reliable alternative to light microscopy. This molecular approach is now being employed in the European project INSIGNIA (https://www.insignia-bee.eu/), which is developing a standard protocol for using the honey bee as a tool for environmental monitoring.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationQuaresma, Andreia; Van der Steen, Joseph; Amaral, Joana S.; Biron, David; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman; Mary, Frances Coffey; Formato, Giovanni; Graaf, Dirk C. De; Gratzer, Kristina; Hatjina, Fani; Kilpinem, Ole; Keller, Alexander; Laget, Dries; Pietropaoli, Marco; Rufino, José; Vejsnaes, Flemming; Pinto, M. Alice (2021). Its2 metabarcoding: a promising approach for identification of botanical origin of bee-collected pollen. In 1st Natural products application: Health, Cosmetic and Food. Bragançapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/28344
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Bragança (IPB)pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleIts2 metabarcoding: a promising approach for identification of botanical origin of bee-collected pollenpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBragançapt_PT
oaire.citation.title1st Natural products application: Health, Cosmetic and Foodpt_PT
person.familyNameQuaresma
person.familyNameAmaral
person.familyNameRufino
person.familyNamePinto
person.givenNameAndreia
person.givenNameJoana S.
person.givenNameJosé
person.givenNameM. Alice
person.identifier.ciencia-id4F1A-4E4A-3F23
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.ciencia-idC414-F47F-6323
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0002-8678-5800
person.identifier.orcid0000-0002-3648-7303
person.identifier.orcid0000-0002-1344-8264
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id57119742600
person.identifier.scopus-author-id55947199100
person.identifier.scopus-author-id8085507800
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationd417b0ac-c8ee-473a-a355-820b5b9a3f55
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication1e24d2ce-a354-442a-bef8-eebadd94b385
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication.latestForDiscoveryd417b0ac-c8ee-473a-a355-820b5b9a3f55

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
resumo-tf01-natural-products-application-2021.pdf
Size:
749.94 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus