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Bioactive properties of dairy beverages functionalized with pure ergosterol and mycosterol extracts: a comparative study with phytosterol counterparts

dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorRudke, Adenilson
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBarros, Lillian
dc.contributor.authorGonçalves, Odinei Hess
dc.contributor.authorRodrigues, Alírio
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2017-01-13T11:23:10Z
dc.date.available2017-01-13T11:23:10Z
dc.date.issued2016
dc.description.abstractAgaricus bisporus L. is the most consumed mushroom worldwide exhibiting a high content of ergosterol in its sterol’s fraction (~90%). Some studies have demonstrated the health benefits of this molecule related to its antioxidant, antitumor and also hypocholesterolemic effects, similar to the ones exhibited by phytosterols. Functional foods containing phytosterols, or their derivatives, can be found at commercial level bearing the European Food Safety Authority approved health claim “able to lower cholesterol levels in serum”. Therefore, this work aims to present the use of mycosterols as an innovative alternative to phytosterols. A. bisporus bioresidues obtained from mushroom’s industry were used to obtain mycosterols by ultrasound assisted extraction. Pure ergosterol and the obtained mycosterol extracts were added to dairy beverages, in a similar amount to the one used in the commercial ones incorporated with phytosterols. All the beverages were analysed immediately (T0) and after seven days of storage at 4ºC (T1) regarding nutritional parameters, antioxidant activity and cytotoxic properties against human tumor cell lines and a non‐tumor porcine liver primary culture. The beverages functionalized with the mycosterols extract revealed an antioxidant activity similar to the commercial beverages with phytosterols. However, beverages with pure ergosterol revealed twice this antioxidant activity. Moreover, only the samples functionalized with pure ergosterol or the mycosterols extract have shown an increase in the antioxidant activity from T0 to T1, meaning that the compound/extracts were able to protect the dairy drink from oxidation, increasing the product shelf life. Samples with pure ergosterol also revealed the highest cytotoxicity for tumor cell lines, while the beverages with phytosterols showed the lowest activity with no significant differences between T0 and T1. All the samples revealed a similar nutritional composition, and none of them revealed toxicity for normal cells. Studies on the hypocholesterolemic effects of the developed beverages are being carried out.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHeleno, Sandrina A.; Rudke, Adenilson; Calhelha, Ricardo C.; Barros, Lillian; Gonçalves, Odinei H.; Rodrigues, A.E.; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2016). Bioactive properties of dairy beverages functionalized with pure ergosterol and mycosterol extracts: a comparative study with phytosterol counterparts. In I International Conference on Research for Sustainable Development in Mountain Regions: Book of Abstracts. Bragança. ISBN 978‐972‐745‐214‐9pt_PT
dc.identifier.isbn978‐972‐745‐214‐9
dc.identifier.urihttp://hdl.handle.net/10198/13768
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleBioactive properties of dairy beverages functionalized with pure ergosterol and mycosterol extracts: a comparative study with phytosterol counterpartspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBragançapt_PT
oaire.citation.titleI International Conference on Research for Sustainable Development in Mountain Regionspt_PT
person.familyNameHeleno
person.familyNameCalhelha
person.familyNameBarros
person.familyNameGonçalves
person.familyNameBarreiro
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameRicardo C.
person.givenNameLillian
person.givenNameOdinei Hess
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8B18-3095-6FC9
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id3418-47D5-5746
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-9528-8187
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridJ-2172-2014
person.identifier.ridJ-3600-2013
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id9335958400
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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