Repository logo
 
Publication

Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication

2018Journal article Open access
Simple item page
dc.contributor.authorSoares, Sónia
dc.contributor.authorGrazina, Liliana
dc.contributor.authorMafra, Isabel
dc.contributor.authorCosta, Joana
dc.contributor.authorPinto, M. Alice
dc.contributor.authorDuc, Hanh Pham
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2018-01-29T10:00:00Z
dc.date.accessioned2018-01-29T10:39:46Z
dc.date.available2018-01-29T10:00:00Z
dc.date.available2018-01-29T10:39:46Z
dc.date.issued2018
dc.description.abstractHoney can be produced by different species of honeybees, with two being of economic importance due to their use in apiculture, namely Apis mellifera (known as European honeybee) and Apis cerana (known as Asian honeybee). Due to the decline of the wild populations of the Asian honeybee, this honey generally attains much higher market value, being prone to adulteration. This work aims at proposing new tools, based on the use of molecular markers, for the entomological authentication of honey. To this end, new species-specific primers were designed targeting the tRNA leu -cox2 intergenic region and allowing the detection of A. cerana DNA by qualitative polymerase chain reaction (PCR). Additionally, a novel real-time PCR method with high resolution melting analysis was developed to target the 16S rRNA gene of both bee species, allowing their discrimination in different clusters. The proposed methodologies were further applied with success in the authentication of Asian and European honey samples by the identification of honeybee DNA, demonstrating the usefulness of these simple and cost-effective new approaches.pt_PT
dc.description.sponsorshipWe thank Matt Webster from Uppsala University for providing the A. cerana samples from China and Thailand. This work was supported by FCT (Fundação para a Ciência e Tecnologia) through project UID/QUI/50006/2013 – POCI/01/0145/FEDER/007265 with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and by the project NORTE-01-0145-FEDER-000011. S. Soares and J. Costa are grateful to FCT grants (SFRH/BD/75091/2010 and SFRH/BPD/102404/2014, respectively) financed by POPH-QREN (subsidised by FSE and MCTES).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSoares, Sónia; Grazina, Liliana; Mafra, Isabel; Costa, Joana; Pinto, M. Alice; Duc, Hanh Pham; Oliveira, M.B.P.P.; Amaral, J.S. (2018). Novel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authentication. Food Research International. ISSN 0963-9969. 105, p. 686-693
dc.identifier.doi10.1016/j.foodres.2017.11.081pt_PT
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/15088
dc.language.isoeng
dc.peerreviewedyespt_PT
dc.relationStructural features and immunoreactivity of plant food allergens: impact of technological food processing and in vitro digestibility
dc.titleNovel diagnostic tools for Asian (Apis cerana) and European (Apis mellifera) honey authenticationen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStructural features and immunoreactivity of plant food allergens: impact of technological food processing and in vitro digestibility
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F75091%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F102404%2F2014/PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameAmaral
person.givenNameM. Alice
person.givenNameJoana S.
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0002-3648-7303
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticlept_PT
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery0667fe04-7078-483d-9198-56d167b19bc5
relation.isProjectOfPublication40f15c30-b6e8-4474-ae1d-d7256c7af84e
relation.isProjectOfPublication24513143-7e25-460c-927a-624699b8b3ca
relation.isProjectOfPublicationf9974285-c431-45c7-8a0c-4f319d06bd3a
relation.isProjectOfPublication.latestForDiscoveryf9974285-c431-45c7-8a0c-4f319d06bd3a

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
8.pdf
Size:
1 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Plain Text
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus