Repository logo
 
Publication

Chemical, cytotoxic, and anti-inflammatory assessment of honey bee venom from Apis mellifera intermissa

2021Journal article Open access
Simple item page
dc.contributor.authorEl Mehdi, Iouraouine
dc.contributor.authorFalcão, Soraia
dc.contributor.authorHarandou, Mustapha
dc.contributor.authorBoujraf, Saïd
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorAnjos, Ofélia
dc.contributor.authorCampos, Maria G.
dc.contributor.authorVilas-Boas, Miguel
dc.date.accessioned2022-04-04T13:59:22Z
dc.date.available2022-04-04T13:59:22Z
dc.date.issued2021
dc.description.abstractThe venom from Apis mellifera intermissa, the main honey bee prevailing in Morocco, has been scarcely studied, despite its known potential for pharmacological applications. In the present work, we investigated the composition, the anti-inflammatory activity, and the venom’s cytotoxic properties from fifteen honey bee venom (HBV) samples collected in three regions: northeast, central, and southern Morocco. The chemical assessment of honey bee venom was performed using LC-DAD/ESI/MSn, NIR spectroscopy and AAS spectroscopy. The antiproliferative effect was evaluated using human tumor cell lines, including breast adenocarcinoma, non-small cell lung carcinoma, cervical carcinoma, hepatocellular carcinoma, and malignant melanoma. Likewise, we assessed the anti-inflammatory activity using the murine macrophage cell line. The study provides information on the honey bee venom subspecies’ main components, such as melittin, apamin, and phospholipase A2, with compositional variation depending on the region of collection. Contents of toxic elements such as cadmium, chromium, and plumb were detected at a concentration below 5 ppm, which can be regarded as safe for pharmaceutical use. The data presented contribute to the first study in HBV from Apis mellifera intermissa and highlight the remarkable antiproliferative and anti-inflammatory effects of HBV, suggesting it to be a candidate natural medicine to explore.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020) and to FRC (UIDB/00239/2020). Thanks to the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project PDR2020-1.0.1-FEADER- 031734: “DivInA-Diversification and Innovation on Beekeeping Production” and project GreenHealth, Norte-01-0145-FEDER-000042. Thanks to the national funding by FCT- Foundation for Science and Technology, through the institutional scientific employment program-contract with Soraia I. Falcão.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEl Mehdi, Iouraouine; Falcão, Soraia; Harandou, Mustapha; Boujraf, Saïd; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Anjos, Ofélia; Campos, Maria G.; Vilas-Boas, Miguel (2021). Chemical, cytotoxic, and anti-inflammatory assessment of honey bee venom from Apis mellifera intermissa. Antibiotics. ISSN 2079-6382. 10:12, p. 1-16pt_PT
dc.identifier.doi10.3390/antibiotics10121514pt_PT
dc.identifier.eissn2079-6382
dc.identifier.urihttp://hdl.handle.net/10198/25328
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationForest Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBee venompt_PT
dc.subjectApis mellifera intermissapt_PT
dc.subjectAnti-inflammatory activitypt_PT
dc.subjectCytotoxic activitypt_PT
dc.subjectChemical compositionpt_PT
dc.subjectNIRpt_PT
dc.titleChemical, cytotoxic, and anti-inflammatory assessment of honey bee venom from Apis mellifera intermissapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleForest Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00239%2F2020/PT
oaire.citation.issue12pt_PT
oaire.citation.startPage1514pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFalcão
person.familyNameCalhelha
person.familyNameFerreira
person.familyNameVilas-Boas
person.givenNameSoraia
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.givenNameMiguel
person.identifier711432
person.identifier144781
person.identifier1693134
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.orcid0000-0003-3735-6951
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-8665-5280
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.ridI-5949-2013
person.identifier.scopus-author-id14832459200
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id6602648497
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5746ab3e-8ffe-4e26-9a41-8392cf031f28
relation.isAuthorOfPublication2d5d1a41-7561-4a01-871c-b4c97da35053
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublicationf58280c8-298b-4e4c-9f7c-4517300d3382
relation.isAuthorOfPublication.latestForDiscovery5746ab3e-8ffe-4e26-9a41-8392cf031f28
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication670ef3ba-f2c2-4857-97f0-1678ff4187de
relation.isProjectOfPublication.latestForDiscovery29718e93-4989-42bb-bcbc-4daff3870b25

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
CEF-antibiotics-10-01514-v2.pdf
Size:
3.17 MB
Format:
Adobe Portable Document Format
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus