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Methanolic extract of Ganoderma lucidum induces autophagy of AGS human gastric tumor cells

2015Journal article Open access
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dc.contributor.authorReis, Filipa S.
dc.contributor.authorLima, Raquel T.
dc.contributor.authorMorales, Patricia
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2015-12-01T10:53:12Z
dc.date.available2015-12-01T10:53:12Z
dc.date.issued2015
dc.description.abstractGanoderma lucidum is one of the most widely studied mushroom species, particularly in what concerns its medicinal properties. Previous studies (including those from some of us) have shown some evidence that the methanolic extract of G. lucidum affects cellular autophagy. However, it was not known if it induces autophagy or decreases the autophagic flux. The treatment of a gastric adenocarcinoma cell line (AGS) with the mushroom extract increased the formation of autophagosomes (vacuoles typical from autophagy). Moreover, the cellular levels of LC3-II were also increased, and the cellular levels of p62 decreased, confirming that the extract affects cellular autophagy. Treating the cells with the extract together with lysossomal protease inhibitors, the cellular levels of LC3-II and p62 increased. The results obtained proved that, in AGS cells, the methanolic extract of G. lucidum causes an induction of autophagy, rather than a reduction in the autophagic flux. To our knowledge, this is the first study proving that statement.pt_PT
dc.description.sponsorshipIPATIMUP integrates the i3S Research Unit, which is partially supported by FCT, the Portuguese Foundation for Science and Technology. This work is funded by FEDER funds through the Operational Programme for Competitiveness Factors-COMPETE and National Funds through the FCT-Foundation for Science and Technology, under the projects “PEst-C/SAU/LA0003/2013”, NORTE-07-0162-FEDER- 00018-Contributos para o reforço da capacidade do IPATIMUP enquanto actor do sistema regional de inovação and NORTE-07-0162-FEDER-000067-Reforço e consolidação da capacidade infraestrutural do IPATIMUP para o sistema regional de inovação, both supported by Programa Operacional Regional do Norte (ON.2-O Novo Norte), through FEDER funds under the Quadro de Referência Estratégico Nacional (QREN). The authors thank COMPETE/QREN/EU for the financial support to CIMO (strategic project PEst-OE/AGR/UI0690/2014) and L. Barros. Authors also thank Foundation for Science and Technology (FCT, Portugal) for R.T. Lima grant (SFRH/BPD/68787/2010) and QREN for the grant of F.S. Reis (NORTE-07-0124-FEDER-000023).pt_PT
dc.identifier.citationReis, Filipa S.; Lima, Raquel T.; Morales, Patricia; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena (2015). Methanolic extract of Ganoderma lucidum induces autophagy of AGS human gastric tumor cells. Molecules. ISSN 1420-3049. 20:10, p. 17872- 17882pt_PT
dc.identifier.doi10.3390/molecules201017872pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10198/12463
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNORTE-07-0162-FEDER- 00018pt_PT
dc.relationNORTE-07-0162-FEDER-000067pt_PT
dc.relationNORTE-07-0124-FEDER-000023pt_PT
dc.relationSMALL MOLECULES AS APOPTOSIS INDUCERS IN CANCER CELLS: INVESTIGATION OF THE MECHANISM OF ACTION
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGanoderma lucidumpt_PT
dc.subjectMethanolic extractpt_PT
dc.subjectAntitumor potentialpt_PT
dc.subjectProgrammed cell death (PCD)pt_PT
dc.subjectAutophagypt_PT
dc.titleMethanolic extract of Ganoderma lucidum induces autophagy of AGS human gastric tumor cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSMALL MOLECULES AS APOPTOSIS INDUCERS IN CANCER CELLS: INVESTIGATION OF THE MECHANISM OF ACTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FSAU%2FLA0003%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68787%2F2010/PT
oaire.citation.endPage17882pt_PT
oaire.citation.startPage17872pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume20pt_PT
oaire.fundingStreamCOMPETE
oaire.fundingStream5876
person.familyNameReis
person.familyNameFerreira
person.givenNameFilipa S.
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-id391F-AFE1-64C7
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-9159-0530
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridI-2093-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id36982144400
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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