Yerba mate as an inexpensive source of analytical standards of chlorogenic acid isomers: an optimization study

Meinhart, Adriana Dillenburg; Silveira, Tayse F. F. da; Godoy, Helena Teixeira

Publication

Yerba mate as an inexpensive source of analytical standards of chlorogenic acid isomers: an optimization study

2023Journal article

dc.contributor.author	Meinhart, Adriana Dillenburg
dc.contributor.author	Silveira, Tayse F. F. da
dc.contributor.author	Godoy, Helena Teixeira
dc.date.accessioned	2024-01-24T14:09:11Z
dc.date.available	2024-01-24T14:09:11Z
dc.date.issued	2023
dc.description.abstract	Chlorogenic acid isomers have been increasingly studied because of their beneficial biological effects in humans. However, their commercial analytical standards are high cost, a fact that limits research. Yerba mate (Ilex paraguariensis) is a lowcost, natural plant matrix with marked contents of chlorogenic acids, but its potential as a source of analytical standards of chlogenic acids has never been investigated. Thus, this study aimed to optimize a method to extract, isolate, and purify analytical standards of six chlorogenic acids (3-caffeoylquinic, 4-caffeoylquinic, 5-caffeoylquinic, 3,4-dicaffeoylquinic, 3,5-dicaffeoylquinic, and 4,5-dicaffeoylquinic) from yerba mate (Ilex paraguariensis) using semi-preparative high-performance liquid chromatography. For this, sequential statistical multivariate approaches (central composite designs) were utilized. Using the optimized extraction conditions (5 mL of ethanol:water 26:74 v/v, stirring for 30 min at 60 °C), 1000 g of yerba mate gave a concentrated extract totalizing 21.57 g of chlorogenic acid isomers. We established optimized chromatographic conditions to obtain analytical standards of each compound individually, as well as to produce a mix containing all the compounds, with high yields and purities above 97%. Thus, the optimized conditions to obtain the standards have an excellent yield, employed a natural low-cost matrix, and used simple and automated processes with potential to produce in laboratory and industrial scale. These results show the potential of yerba mate as a novel source of standards of chlorogenic acids, and provide an effective method to produce them in laboratories worldwide, which may contribute to advance the research on these compounds.	pt_PT
dc.description.sponsorship	This work was supported by CAPES (Coordination for the Improvement of Higher Education Personnel), Project numbers 001 and 1267386.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Meinhart, Adriana Dillenburg; Silveira, Tayse Ferreira; Godoy, Helena Teixeira (2023). Yerba mate as an inexpensive source of analytical standards of chlorogenic acid isomers: an optimization study. Food Analytical Methods. ISSN 1936-9751. 17:1, p. 83-95	pt_PT
dc.identifier.doi	10.1007/s12161-023-02549-7	pt_PT
dc.identifier.eissn	1936-976X
dc.identifier.issn	1936-9751
dc.identifier.uri	http://hdl.handle.net/10198/29358
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Springer Nature	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Semi-preparative HPLC	pt_PT
dc.subject	Ethanol	pt_PT
dc.subject	Fast	pt_PT
dc.subject	Low cost	pt_PT
dc.subject	High yield	pt_PT
dc.subject	Purity	pt_PT
dc.title	Yerba mate as an inexpensive source of analytical standards of chlorogenic acid isomers: an optimization study	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	13	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	Food Analytical Methods	pt_PT
person.familyName	Silveira
person.givenName	Tayse F. F. da
person.identifier.ciencia-id	541E-97D4-A3E5
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	article	pt_PT
relation.isAuthorOfPublication	6b60de6a-591c-4a30-95ea-cc5feebe8a30
relation.isAuthorOfPublication.latestForDiscovery	6b60de6a-591c-4a30-95ea-cc5feebe8a30