Publication
Extraction and detection of mycotoxins in medicinal and aromatic plants: a case study with Aloysia citrodora P.
| dc.contributor.author | Pereira, Eliana | |
| dc.contributor.author | Ferreira, Isabel C.F.R. | |
| dc.contributor.author | Rodrigues, Paula | |
| dc.date.accessioned | 2016-09-21T10:08:56Z | |
| dc.date.available | 2016-09-21T10:08:56Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Plants frequently suffer contaminations by toxigenic fungi, and their mycotoxins can be produced throughout growth, harvest, drying and storage periods. The objective of this work was to validate a method for detection of toxins in medicinal and aromatic plants, through a fast and highly sensitive method, optimizing the joint co-extraction of aflatoxins (AF: AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) by using Aloysia citrodora P. (lemon verbena) as a case study. For optimization purposes, samples were spiked (n=3) with standard solutions of a mix of the four AFs and OTA at 10 ng/g for AFB1, AFG1 and OTA, and at 6 ng/g of AFB2 and AFG2. Several extraction procedures were tested: i) ultrasound-assisted extraction in sodium chloride and methanol/water (80:20, v/v) [(OTA+AFs)1]; ii) maceration in methanol/1% NaHCO3 (70:30, v/v) [(OTA+AFs)2]; iii) maceration in methanol/1% NaHCO3 (70:30, v/v) (OTA1); and iv) maceration in sodium chloride and methanol/water (80:20, v/v) (AF1). AF and OTA were purified using the mycotoxin-specific immunoaffinity columns AflaTest WB and OchraTest WB (VICAM), respectively. Separation was performed with a Merck Chromolith Performance C18 column (100 x 4.6 mm) by reverse-phase HPLC coupled to a fluorescence detector (FLD) and a photochemical derivatization system (for AF). The recoveries obtained from the spiked samples showed that the single-extraction methods (OTA1 and AF1) performed better than co-extraction methods. For in-house validation of the selected methods OTA1 and AF1, recovery and precision were determined (n=6). The recovery of OTA for method OTA1 was 81%, and intermediate precision (RSDint) was 1.1%. The recoveries of AFB1, AFB2, AFG1 and AFG2 ranged from 64% to 110% for method AF1, with RSDint lower than 5%. Methods OTA1 and AF1 showed precision and recoveries within the legislated values and were found to be suitable for the extraction of OTA and AF for the matrix under study. | pt_PT |
| dc.identifier.citation | Pereira, Eliana; Ferreira, Isabel C.F.R.; Rodrigues, Paula (2016). Extraction and detection of mycotoxins in medicinal and aromatic plants: a case study with Aloysia citrodora P. In International Conference on Food Contaminants: challenges in chemical mixtures. Lisboa. ISBN 978-989-8794-20-8 | pt_PT |
| dc.identifier.isbn | 978-989-8794-20-8 | |
| dc.identifier.uri | http://hdl.handle.net/10198/13273 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Extraction and detection of mycotoxins in medicinal and aromatic plants: a case study with Aloysia citrodora P. | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT | |
| oaire.citation.conferencePlace | Lisboa, Portugal | pt_PT |
| oaire.citation.title | ICFC2015 International Conference on Food Contaminants: challenges in chemical mixtures | pt_PT |
| oaire.fundingStream | 5876 | |
| person.familyName | Pereira | |
| person.familyName | Ferreira | |
| person.familyName | Rodrigues | |
| person.givenName | Eliana | |
| person.givenName | Isabel C.F.R. | |
| person.givenName | Paula | |
| person.identifier | 144781 | |
| person.identifier.ciencia-id | BE17-462D-9A75 | |
| person.identifier.ciencia-id | 9418-CF95-9919 | |
| person.identifier.ciencia-id | 2312-18AE-DA42 | |
| person.identifier.orcid | 0000-0003-2854-6745 | |
| person.identifier.orcid | 0000-0003-4910-4882 | |
| person.identifier.orcid | 0000-0002-3789-2730 | |
| person.identifier.rid | Q-8017-2018 | |
| person.identifier.rid | E-8500-2013 | |
| person.identifier.scopus-author-id | 55938912300 | |
| person.identifier.scopus-author-id | 36868826600 | |
| person.identifier.scopus-author-id | 7102639646 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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