Publicação
Viability of Beauveria bassiana isolates after storage under several preservation methods
| dc.contributor.author | Oliveira, Ivo | |
| dc.contributor.author | Pereira, J.A. | |
| dc.contributor.author | Bento, Albino | |
| dc.contributor.author | Baptista, Paula | |
| dc.date.accessioned | 2014-09-22T08:41:41Z | |
| dc.date.available | 2014-09-22T08:41:41Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | Beauveria bassiana is a worldwide distributed entomopathogenic fungus, which is used nowadays as an alternative to chemical pesticides in the control of several agriculture pests. Therefore, a preservation method must be established in order to maintain fungal culture and stocks, while fungal characteristics, like morphological features, spore production and viability, are kept well preserved. Although a large number of different storage methods are available and described, each fungus presents different characteristics, and therefore a new challenge for its preservation. In this work, we evaluate the effect of 1-year preservation by lyophilization, glycerol-freeze at −20°C and sub-culturing on growth, production and viability of spores and macro- and micro-morphology of three isolates of B. bassiana. Overall, results indicate that sub-culturing is the best method for conservation of this fungal species. However, if long-term storage is required, glycerol-freeze proved to be the most capable method of preservation. | por |
| dc.description.sponsorship | PTCD/AGR-AAM/102600/2008 | por |
| dc.identifier.citation | Oliveira, Ivo; Pereira, J.A.; Bento, Albino; Baptista, Paula (2011). Viability of Beauveria bassiana isolates after storage under several preservation methods. Annals of Microbiology. ISSN 1590-4261. 61:2, p. 339-344 | por |
| dc.identifier.doi | 10.1007/s13213-010-0147-8 | |
| dc.identifier.issn | 1590-4261 | |
| dc.identifier.uri | http://hdl.handle.net/10198/10512 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Springer | por |
| dc.relation | Entomopathogenic fungi associated to olive pests: isolation, characterization and selection for biological control | |
| dc.subject | Beauveria bassiana | por |
| dc.subject | Preservation | por |
| dc.subject | Fungus morphology | por |
| dc.subject | Viability | por |
| dc.title | Viability of Beauveria bassiana isolates after storage under several preservation methods | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | PTDC/AGR-AAM/102600/2008 | |
| oaire.awardNumber | SFRH/BD/44265/2008 | |
| oaire.awardTitle | Entomopathogenic fungi associated to olive pests: isolation, characterization and selection for biological control | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-AAM%2F102600%2F2008/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44265%2F2008/PT | |
| oaire.citation.endPage | 344 | por |
| oaire.citation.issue | 2 | por |
| oaire.citation.startPage | 339 | por |
| oaire.citation.title | Annals of Microbiology | por |
| oaire.citation.volume | 61 | por |
| oaire.fundingStream | 5876-PPCDTI | |
| oaire.fundingStream | SFRH | |
| person.familyName | Pereira | |
| person.familyName | Bento | |
| person.familyName | Baptista | |
| person.givenName | José Alberto | |
| person.givenName | Albino | |
| person.givenName | Paula | |
| person.identifier.ciencia-id | 611F-80B2-A7C1 | |
| person.identifier.ciencia-id | D516-325A-9AD7 | |
| person.identifier.ciencia-id | 7D11-FE1E-CD0F | |
| person.identifier.orcid | 0000-0002-2260-0600 | |
| person.identifier.orcid | 0000-0001-5215-785X | |
| person.identifier.orcid | 0000-0001-6331-3731 | |
| person.identifier.rid | L-6798-2014 | |
| person.identifier.rid | N-9706-2016 | |
| person.identifier.scopus-author-id | 57204366348 | |
| person.identifier.scopus-author-id | 35247694000 | |
| person.identifier.scopus-author-id | 14051688000 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
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