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Viability of Beauveria bassiana isolates after storage under several preservation methods

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dc.contributor.authorOliveira, Ivo
dc.contributor.authorPereira, J.A.
dc.contributor.authorBento, Albino
dc.contributor.authorBaptista, Paula
dc.date.accessioned2014-09-22T08:41:41Z
dc.date.available2014-09-22T08:41:41Z
dc.date.issued2011
dc.description.abstractBeauveria bassiana is a worldwide distributed entomopathogenic fungus, which is used nowadays as an alternative to chemical pesticides in the control of several agriculture pests. Therefore, a preservation method must be established in order to maintain fungal culture and stocks, while fungal characteristics, like morphological features, spore production and viability, are kept well preserved. Although a large number of different storage methods are available and described, each fungus presents different characteristics, and therefore a new challenge for its preservation. In this work, we evaluate the effect of 1-year preservation by lyophilization, glycerol-freeze at −20°C and sub-culturing on growth, production and viability of spores and macro- and micro-morphology of three isolates of B. bassiana. Overall, results indicate that sub-culturing is the best method for conservation of this fungal species. However, if long-term storage is required, glycerol-freeze proved to be the most capable method of preservation.por
dc.description.sponsorshipPTCD/AGR-AAM/102600/2008por
dc.identifier.citationOliveira, Ivo; Pereira, J.A.; Bento, Albino; Baptista, Paula (2011). Viability of Beauveria bassiana isolates after storage under several preservation methods. Annals of Microbiology. ISSN 1590-4261. 61:2, p. 339-344por
dc.identifier.doi10.1007/s13213-010-0147-8
dc.identifier.issn1590-4261
dc.identifier.urihttp://hdl.handle.net/10198/10512
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationEntomopathogenic fungi associated to olive pests: isolation, characterization and selection for biological control
dc.subjectBeauveria bassianapor
dc.subjectPreservationpor
dc.subjectFungus morphologypor
dc.subjectViabilitypor
dc.titleViability of Beauveria bassiana isolates after storage under several preservation methodspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEntomopathogenic fungi associated to olive pests: isolation, characterization and selection for biological control
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-AAM%2F102600%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44265%2F2008/PT
oaire.citation.endPage344por
oaire.citation.issue2por
oaire.citation.startPage339por
oaire.citation.titleAnnals of Microbiologypor
oaire.citation.volume61por
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamSFRH
person.familyNamePereira
person.familyNameBento
person.familyNameBaptista
person.givenNameJosé Alberto
person.givenNameAlbino
person.givenNamePaula
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.ciencia-idD516-325A-9AD7
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.orcid0000-0002-2260-0600
person.identifier.orcid0000-0001-5215-785X
person.identifier.orcid0000-0001-6331-3731
person.identifier.ridL-6798-2014
person.identifier.ridN-9706-2016
person.identifier.scopus-author-id57204366348
person.identifier.scopus-author-id35247694000
person.identifier.scopus-author-id14051688000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication233115be-9d46-49d0-8b7d-2d64406d64a0
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relation.isAuthorOfPublication.latestForDiscovery233115be-9d46-49d0-8b7d-2d64406d64a0
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