Publication
Photoinactivation of sulfate-reducing bacteria using 1,9-dimethyl-methylene blue – DMMB and laser light
| datacite.subject.fos | Engenharia e Tecnologia::Engenharia dos Materiais | |
| datacite.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | |
| datacite.subject.sdg | 09:Indústria, Inovação e Infraestruturas | |
| dc.contributor.author | Santos, Gustavo Vital dos | |
| dc.contributor.author | Moura, Hesrom Fernandes Serra | |
| dc.contributor.author | Pedro Jorge Louro Crugeira | |
| dc.contributor.author | Anna Paula Lima Teixeira da Silva | |
| dc.contributor.author | Isabele Cardoso Vieira de Castro | |
| dc.contributor.author | Wellington Luís Reis Costa | |
| dc.contributor.author | Paulo Fernando de Almeida | |
| dc.contributor.author | Antonio Luiz Barbosa Pinheiro | |
| dc.contributor.author | Crugeira, Pedro | |
| dc.date.accessioned | 2025-02-28T15:07:05Z | |
| dc.date.available | 2025-02-28T15:07:05Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Annually, the oil and gas industry faces equipment losses and product quality degradation due to the presence of sulfate-reducing bacteria (SRB). Given the negative impact of SRB, this study evaluates the use of photoinactivation (PI) with zinc chloride double salt of 1,9-Dimethyl-Methylene Blue (DMMB) as a photosensitizer (PS) in varying concentrations and combined with Laser light at different exposures in an SRB consortium. For culture growth, a modified Postgate C medium (without ferrous sulfate) was used, and cell quantification was performed on 100 μL aliquots of the consortium, read on a spectrophotometer (λ600 nm) in an oxygen- and light-free environment at room temperature. Statistical analyses included two-way ANOVA and ANOVA with interaction to separately and jointly evaluate the effects of PS and light in PI. Results indicated microbial activity in all groups, with an antimicrobial inhibition rate exceeding 50 % (p < 0.05) for concentrations above 1.5 μg/mL of DMMB. PI efficacy significantly depended on DMMB concentration and light density, achieving a 70.58 % (55.73–70.58, with a mean of 66.71 %) reduction (p < 0.05) with 1.5 μg/mL of DMMB and a 70.15 % (65–70.15, with a mean of 68.21 %) reduction with 2.0 μg/mL at an intensity of 21.6 J/cm2. In conclusion, PI presents a promising alternative to biocides in the oil and gas industry, offering easy application, avoiding bacterial resistance, being environmentally safe, and compatible with other SRB population control techniques. | por |
| dc.description.sponsorship | Authors G. V. dos Santos thank the Fundaç˜ao de Amparo a Pesquisa do Estado da Bahia (Fabesb) for an MSci Grant (BOL0426/2021) and A. P. L.T. da Silva for a PhD Grant (BOL0667/2021). Authors P. F. de Almeida and A. L. B. Pinheiro would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research grants (respectively 302753/2020-6 and 304279/2018-8) | |
| dc.identifier.doi | 10.1016/j.jphotobiol.2025.113103 | |
| dc.identifier.issn | 1011-1344 | |
| dc.identifier.uri | http://hdl.handle.net/10198/34324 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.ispartof | Journal of Photochemistry and Photobiology B: Biology | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Antimicrobial activity | |
| dc.subject | Biocides | |
| dc.subject | Laser | |
| dc.subject | Photoinactivation | |
| dc.subject | SRB | |
| dc.title | Photoinactivation of sulfate-reducing bacteria using 1,9-dimethyl-methylene blue – DMMB and laser light | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 9 | |
| oaire.citation.startPage | 1 | |
| oaire.citation.title | Journal of Photochemistry and Photobiology B: Biology | |
| oaire.citation.volume | 263 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Crugeira | |
| person.givenName | Pedro | |
| person.identifier.ciencia-id | 8F17-E60C-A45D | |
| person.identifier.orcid | 0000-0002-7084-3711 | |
| relation.isAuthorOfPublication | ce777646-e21d-4491-a8a8-345efc9517a1 | |
| relation.isAuthorOfPublication.latestForDiscovery | ce777646-e21d-4491-a8a8-345efc9517a1 |